|
| Status |
Public on Jan 01, 2023 |
| Title |
M_fresh_4A |
| Sample type |
genomic |
| |
|
| Source name |
Term placenta
|
| Organism |
Mus musculus |
| Characteristics |
tissue: placenta
fetal gender: Male
developmental stage: E18.5
group: fresh
|
| Treatment protocol |
Placentas were collected at E18.5, snap frozen in liquid nitrogen and stored at -80C for DNA extraction.
|
| Growth protocol |
All animals were housed in polysulfone cages containing drinking water and chow (Laboratory Irradiated Rodent Diet 5LG4, LabDiet) ad libitum.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted and purified from Placentas samples using Phenol:Chloroform according to lab protocols. Then samples were Bisulfite-treated using Qiagen EpiTect Bisulfite Conversion Kit.
|
| Label |
Cy3, Cy5
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Mouse Methylation Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Data processing |
Sesame R package
|
| |
|
| Submission date |
Feb 10, 2022 |
| Last update date |
Jan 01, 2023 |
| Contact name |
Yemin Lan |
| Organization name |
University of Pennsylvania
|
| Street address |
3400 Civic Center Blvd
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL31191 |
| Series (1) |
| GSE196547 |
Embryo Cryopreservation leads to sex-specific DNA methylation perturbations in both human and mouse placentas |
|
