|
Status |
Public on Dec 19, 2023 |
Title |
Postransplant lymphoproliferative disorder (PTLD) 20 |
Sample type |
genomic |
|
|
Source name |
LN, intestinal
|
Organism |
Homo sapiens |
Characteristics |
disease state: PTLD morphology: Monomorphic PTLD diagnosis: DLBCL gender: male tissue preparation: FFPE
|
Treatment protocol |
none
|
Growth protocol |
none
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA from formalin-fixed paraffin-embedded (FFPE) was extracted using AllPrep DNA/RNA FFPE Qiagen extraction kit (Qiagen, Hilden, Germany)
|
Label |
Biotin
|
Label protocol |
Affymetrix OncoScan™ FFPE Assay kit /GPL18602
|
|
|
Hybridization protocol |
Samples were hybridized onto OncosCan Array for 16h at 49º using Affymetrix Hybridization oven.
|
Scan protocol |
Affymetrix® GeneChip® Scanner 3000G to detect target bound to Oncoscan array after staining with Biotin.
|
Description |
Hybridized to Affymetrix OncoScan FFPE Assay
|
Data processing |
Nexus biodiscovery software Gains and losses were evaluated with visual inspection by 2 different observers using Nexus Biodiscovery software (Biodiscovery, Hawthorne CA, USA). The CNAs with a minimum size of 100kb were considered real. Loss of heterozigosity regions where considered when size was >5Mb.
|
|
|
Submission date |
Mar 09, 2022 |
Last update date |
Dec 19, 2023 |
Contact name |
Itziar Salaverria |
E-mail(s) |
isalaver@clinic.cat
|
Organization name |
Hospital Clínic
|
Street address |
Rossello 153
|
City |
Barcelona |
ZIP/Postal code |
08036 |
Country |
Spain |
|
|
Platform ID |
GPL18602 |
Series (1) |
GSE198253 |
Unravelling the heterogeneous molecular landscape of pediatric post-transplant lymphoproliferative disorders |
|