|
| Status |
Public on Mar 23, 2015 |
| Title |
Rt_h_treated_3 |
| Sample type |
RNA |
| |
|
| Source name |
Male rat heart, treated with amiodarone
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
gender: male
age: 11 weeks
weight: 290-320 grams
tissue: heart, left ventricle
treatment: amiodarone
|
| Treatment protocol |
Male Sprague-Dawley (CD) rats, which were 10 weeks old and weighed 290-320 grams at the beginning of the study, were treated for ten days with amiodarone by oral route at a dose of 300mg/Kg/die. The control group received the vehicle (methylcellulose 1%, w/v).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Heart RNA was extracted according to the Qiagen RNA easy MiniKit protocol for tissues samples, while blood RNA was extracted according to the Qiagen PAXgene Blood RNA kit.
|
| Label |
Cy3
|
| Label protocol |
Amplified and labeled according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol.
|
| |
|
| Hybridization protocol |
Hybridized according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol.
|
| Scan protocol |
Array was scanned using the Agilent scanner G2565BA according to the standard Agilent protocol at a resolution of 5 microns, modifying the default setting to scan the slide twice at two different sensitivity settings (XDRHi 100% and XDRLo 10%).
|
| Description |
Rn_6526_002
Heart sample collected from male rat, submerged in RNAlater and stored at -20°C until extraction.
|
| Data processing |
Data processed with FeatureExtraction v9.5 and normalized using R2.9.2 statistical software.
|
| |
|
| Submission date |
Sep 18, 2010 |
| Last update date |
Mar 24, 2015 |
| Contact name |
Mery Giantin |
| E-mail(s) |
mery.giantin@unipd.it
|
| Phone |
+39 049 8272935
|
| Organization name |
University of Padova
|
| Department |
Dip. Biomedicina Comparata e Alimentazione
|
| Lab |
Farmacologia e Tossicologia
|
| Street address |
Viale Università , 16
|
| City |
Agripolis - Legnaro (PD) |
| State/province |
Padova |
| ZIP/Postal code |
35020 |
| Country |
Italy |
| |
|
| Platform ID |
GPL4135 |
| Series (1) |
| GSE24213 |
Expression profiling of rat heart and blood after amiodarone treatment |
|
