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Sample GSM5956243 Query DataSets for GSM5956243
Status Public on Apr 01, 2022
Title plasma_exosome_control_rep5
Sample type RNA
 
Source name plasma exosome, healthy control
Organism Rattus norvegicus
Characteristics strain: Wistar
gender: Male
tissue: plasma exosome
condition: healthy control
Treatment protocol Wistar rats were subjected to four cycles of remote ischemia, and each cycle consisted of 5-minute ischemia and 5-minute reperfusion.
Extracted molecule total RNA
Extraction protocol Exosomes were isolated from plasma using a previously reported method (Wang et al., 2018). We collected venous blood (10 ml) from each participant using an EDTA-anticoagulated tube. Exosomes were isolated via differential centrifugation using ultracentrifuge (Beckman) and were stored at -80°C. Total RNA was isolated using TRIzol reagent (Life Technologies, Carlsbad, CA, USA) and purified with an RNeasy Mini Kit (Qiagen, Valencia, CA, USA).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-8 ug total RNA
 
Hybridization protocol Following fragmentation, 10ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Mouse Genome 430 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol Genechips were scanned by GeneChip® Scanner 3000.
Description Gene expression data from healthy control
Data processing The data were analyzed with Expression Console™, Version 1.3.1.
 
Submission date Mar 16, 2022
Last update date Apr 02, 2022
Contact name Xinhui Fan
E-mail(s) fanxinhui1030@126.com
Phone 18366111002
Organization name Qilu Hospital of Shandong University
Street address 107 Wenhuaxi Road
City Jinan
State/province Shandong Province
ZIP/Postal code 250012
Country China
 
Platform ID GPL22388
Series (1)
GSE198747 Expression data from plasma exosomal microRNA of rats with remote ischemic conditioning and normal controls

Data table header descriptions
ID_REF
VALUE signal intensity

Data table
ID_REF VALUE
TC1300002007.rn.1 3.49
TC0400000034.rn.1 3.57
TC0100003962.rn.1 3.66
TC1100001218.rn.1 3.82
TC0100000051.rn.1 3.78
TC0300003242.rn.1 3.82
TC0600001687.rn.1 3.59
TC1300000228.rn.1 5
TC1700000380.rn.1 4.04
TC0900002253.rn.1 4.11
TC1400002150.rn.1 11.07
TC1700001741.rn.1 3.9
TC0100007309.rn.1 4.31
TC0600000444.rn.1 3.73
TC1100001039.rn.1 5.05
TC0100007626.rn.1 3.97
TC0100005611.rn.1 4.07
TC1000000748.rn.1 3.59
TC0500003420.rn.1 10.11
TC1700001833.rn.1 5.66

Total number of rows: 68842

Table truncated, full table size 1544 Kbytes.




Supplementary file Size Download File type/resource
GSM5956243_Control_5_RTA-1_0.CEL.gz 17.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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