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Status |
Public on Apr 01, 2022 |
Title |
plasma_exosome_control_rep5 |
Sample type |
RNA |
|
|
Source name |
plasma exosome, healthy control
|
Organism |
Rattus norvegicus |
Characteristics |
strain: Wistar gender: Male tissue: plasma exosome condition: healthy control
|
Treatment protocol |
Wistar rats were subjected to four cycles of remote ischemia, and each cycle consisted of 5-minute ischemia and 5-minute reperfusion.
|
Extracted molecule |
total RNA |
Extraction protocol |
Exosomes were isolated from plasma using a previously reported method (Wang et al., 2018). We collected venous blood (10 ml) from each participant using an EDTA-anticoagulated tube. Exosomes were isolated via differential centrifugation using ultracentrifuge (Beckman) and were stored at -80°C. Total RNA was isolated using TRIzol reagent (Life Technologies, Carlsbad, CA, USA) and purified with an RNeasy Mini Kit (Qiagen, Valencia, CA, USA).
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-8 ug total RNA
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|
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Hybridization protocol |
Following fragmentation, 10ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Mouse Genome 430 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
Genechips were scanned by GeneChip® Scanner 3000.
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Description |
Gene expression data from healthy control
|
Data processing |
The data were analyzed with Expression Console™, Version 1.3.1.
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|
|
Submission date |
Mar 16, 2022 |
Last update date |
Apr 02, 2022 |
Contact name |
Xinhui Fan |
E-mail(s) |
fanxinhui1030@126.com
|
Phone |
18366111002
|
Organization name |
Qilu Hospital of Shandong University
|
Street address |
107 Wenhuaxi Road
|
City |
Jinan |
State/province |
Shandong Province |
ZIP/Postal code |
250012 |
Country |
China |
|
|
Platform ID |
GPL22388 |
Series (1) |
GSE198747 |
Expression data from plasma exosomal microRNA of rats with remote ischemic conditioning and normal controls |
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