|
Status |
Public on May 01, 2011 |
Title |
heart_TAC1w_rep2 |
Sample type |
RNA |
|
|
Source name |
mouse wildtype ventricle
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 tissue: heart ventricle gender: male stress: transverse aortic constriction time: 1 week
|
Treatment protocol |
The wile-type mice were subject to sham- or TAC-operation.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared from total RNA as described by Affymetrix.
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|
|
Hybridization protocol |
RNA samples were hybridizaed with Affymetrix expression arrays offering whole-transcript coverage. After hybridization, non-specific binding was washed off according to the manufacturer's protocol.
|
Scan protocol |
Affymetrix arrays were scanned with the Affymetrix GeneArray scanner.
|
Description |
1week pressure overload BTIA_EXON_TAC2_053008.CEL
|
Data processing |
In the Expression Console software, hybridization intensity data were normalized using the Robust Multichip Average (RMA) method.
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|
|
Submission date |
Sep 21, 2010 |
Last update date |
May 01, 2011 |
Contact name |
Jiyeon Park |
E-mail(s) |
parkji7@snu.ac.kr
|
Organization name |
Seoul National Univerisy
|
Department |
Biomedical Informatics
|
Lab |
Bioinformatics
|
Street address |
103 Daehak-ro, Jongno-gu
|
City |
Seoul |
ZIP/Postal code |
110-799 |
Country |
South Korea |
|
|
Platform ID |
GPL6096 |
Series (1) |
GSE24242 |
Gene and exon expression change in cardiac hypertrophy with pressure overload |
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