|
| Status |
Public on Mar 01, 2023 |
| Title |
WT_D1_4 |
| Sample type |
RNA |
| |
|
| Source name |
Nucleus accumbens
|
| Organism |
Rattus norvegicus |
| Characteristics |
genotype: AAV-DIO-L10aGFP
cell type: D1
batch: 1
Sex: m
|
| Treatment protocol |
Rats were injected in the NAc with either AAV-DIO-L10aGFP or a 50-50 mixture of AAV-HDAC5-3SA and AAV-L10aGFP. After about 4 weeks of expression, NAc tissue was harvested using native GFP fluorescence as a guide
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from the Ribo-TRAP assay were submitted to the Medical University of South Carolina Proteogenomics Facility for DNA microarray analysis
|
| Label |
biotin
|
| Label protocol |
biotin-labeled cDNA using 90 ng of total RNA and the GeneChip™ WT PLUS Reagent Kit
|
| |
|
| Hybridization protocol |
Hybridizations overnight to Mouse Clariom D microarrays (ThermoFisher) and post-hybridization washing, and staining were done with GeneChip™ Hybridization, Wash, and Stain Kit, Fluidics Station 450.
|
| Scan protocol |
Scans were done on 7G scanner according to manufacturer protocols
|
| Data processing |
Microarray data were analyzed with custom codes in R.
|
| |
|
| Submission date |
Apr 01, 2022 |
| Last update date |
Mar 01, 2023 |
| Contact name |
Stefano Berto |
| E-mail(s) |
berto@musc.edu
|
| Phone |
4696626889
|
| Organization name |
Medical University of South Carolina
|
| Department |
Neuroscience
|
| Lab |
BE208
|
| Street address |
68 President Street
|
| City |
Charleston |
| State/province |
SC |
| ZIP/Postal code |
29403 |
| Country |
USA |
| |
|
| Platform ID |
GPL22388 |
| Series (1) |
| GSE199985 |
Epigenetic function during heroin self-administration controls future relapse-like behaviors in a cell type-specific manner |
|
