|
| Status |
Public on Sep 28, 2010 |
| Title |
ICM fraction 19 |
| Sample type |
genomic |
| |
|
| Source name |
ICM fraction
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: inner cell mass
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
We thawed all fractions at 22°C, and then added Arcturus PicoPure Lysis Buffer (Molecular Devices, Sunnyvale, CA) to each of the biopsies. The tubes were incubated at 56°C for one hour, and then heat inactivated at 95°C for 10 minutes. DNA from the lysed biospsies was amplified using a commercial kit (GE Healthcare, Waukesha, WI) for multiple displacement amplification (MDA). MDA reactions were incubated at 30°C for 2.5 hours and then heat-inactivated at 65°C for five minutes.
|
| Label |
C-Bio and A-DNP
|
| Label protocol |
as per Illumina's instructions except reduced amplification time to 6 hours
|
| |
|
| Hybridization protocol |
as per Illumina's instructions except reduced time to 6 hours
|
| Scan protocol |
iScan scanner
|
| Description |
4948721054_R03C02
|
| Data processing |
Genotypes are inherently inaccurate in single cell microarray data due to biases introduced during multiple displacement amplification. Therefore genotypes were not produced, and instead raw intensity data was used to build probabilistic models under the framework of the Parental Support algorithm.
|
| |
|
| Submission date |
Sep 27, 2010 |
| Last update date |
Sep 28, 2010 |
| Contact name |
David Scott Johnson |
| E-mail(s) |
seasquirtdoctor@gmail.com
|
| Phone |
650-725-3018
|
| Organization name |
Stanford University
|
| Department |
Genetics
|
| Lab |
Richard M. Myers
|
| Street address |
300 Pasteur Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL6985 |
| Series (1) |
| GSE22864 |
Comprehensive Analysis of Karyotypic Mosaicism between Trophectoderm and Inner Cell Mass |
|
