|
| Status |
Public on Dec 06, 2005 |
| Title |
tumor immune evasion - P3 cell line-replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
mouse cancer cell line, P3
|
| Organism |
Mus musculus |
| Characteristics |
strain - C57BL/6
gender - female
tissue - P0/TC-1 is a cancer cell line derived from lung fibroblasts. P3 is derived from P0.
|
| Treatment protocol |
P3 is derived from P0 through 3 rounds of in vivo immune selection. P3 is a highly immune resistant cell line.
|
| Growth protocol |
The cell line was cultured in vitro in 10-cm dishes until 70-75% confluency and then total RNA was isolated.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIzol reagent (Invitrogen), followed by a clean-up step using RNeasy mini kit (Qiagen).
|
| Label |
biotin-labeled cRNA, detected with streptavidin phycoerythin conjugate
|
| |
|
| Description |
This is one of 4 biological replicates for P3 cell line.
|
| Data processing |
background correction made with RMA, Quantile Normalization, GC-RMA
|
| |
|
| Submission date |
Jun 06, 2005 |
| Last update date |
Jun 07, 2005 |
| Contact name |
Ken-Yu Lin |
| E-mail(s) |
kenyulin@jhmi.edu
|
| Phone |
410-614-4906
|
| Organization name |
Johns Hopkins University School of Medicine
|
| Department |
Pathology
|
| Lab |
T.-C. Wu
|
| Street address |
720 Rutland Ave, Ross 512
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE2774 |
Identification of tumor immune evasion mechanism using P0 and P3 cell lines |
|
