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Sample GSM6047841 Query DataSets for GSM6047841
Status Public on Aug 10, 2022
Title 20210827_AOberst-20210826-01_1_01
Sample type RNA
 
Source name mouse embryonic fibroblasts
Organism Mus musculus
Characteristics cell type: mouse embryonic fibroblasts
genotype: MLKLko
sirna: siNT
Treatment protocol 5x10^5 cells were transfected with siRNA using Dharmafect reagent for 24 hours.
Growth protocol Cell were grown in complete DMEM medium in 12 well plates.
Extracted molecule total RNA
Extraction protocol Cell were collected in TRIzol reageatnt and total RNA was extracted by phenol-chloroform phase separation followed by RNA precipitation according to the manufacturer's protocol.
Label Capture probe-3'biotin, Reporter probe-5' barcode signal
Label protocol NanoString nCounter XT gene expression assay protocol
 
Hybridization protocol NanoString nCounter XT gene expression assay protocol
Scan protocol N/A
Description Gene Expression CodeSet profiling 254 genes; 248 inflammation-related mouse genes and 6 housekeeping genes.
Data processing Data was normalized, quantified, and analyzed using the nSolver software (Nanostring Technologies Inc., Seattle, WA) with default parameters to noramlize data to housekeeping genes.
 
Submission date Apr 18, 2022
Last update date Aug 10, 2022
Contact name Andrew Oberst
E-mail(s) oberst@uw.edu
Organization name University of Washington
Street address 750 Republican street
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL20885
Series (1)
GSE200985 ADAR1 mutation causes ZBP1-dependent immunopathology I

Data table header descriptions
ID_REF
VALUE normalized signal

Data table
ID_REF VALUE
Ager 3.305576472
Alox12 1.305576472
Alox15 3.890538973
Alox5 3.305576472
Areg 7.175941192
Arg1 2.890538973
Atf2 9.333482469
Bcl2l1 9.414100929
Bcl6 4.949432662
Birc2 7.920286316
C1qa 3.112931394
C1qb 3.305576472
C1ra 5.393039313
C2 3.112931394
C3 1.890538973
C3ar1 5.475501474
C4a 5.663128477
C6 2.890538973
C7 3.305576472
C8a 3.112931394

Total number of rows: 248

Table truncated, full table size 4 Kbytes.




Supplementary file Size Download File type/resource
GSM6047841_20210827_AOberst-20210826-01_1_01.RCC.gz 3.1 Kb (ftp)(http) RCC
Processed data included within Sample table

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