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Status |
Public on Aug 10, 2022 |
Title |
20210827_AOberst-20210826-01_7_07 |
Sample type |
RNA |
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Source name |
mouse embryonic fibroblasts
|
Organism |
Mus musculus |
Characteristics |
cell type: mouse embryonic fibroblasts genotype: MLKLko sirna: siADAR
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Treatment protocol |
5x10^5 cells were transfected with siRNA using Dharmafect reagent for 24 hours.
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Growth protocol |
Cell were grown in complete DMEM medium in 12 well plates.
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Extracted molecule |
total RNA |
Extraction protocol |
Cell were collected in TRIzol reageatnt and total RNA was extracted by phenol-chloroform phase separation followed by RNA precipitation according to the manufacturer's protocol.
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Label |
Capture probe-3'biotin, Reporter probe-5' barcode signal
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Label protocol |
NanoString nCounter XT gene expression assay protocol
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|
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Hybridization protocol |
NanoString nCounter XT gene expression assay protocol
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Scan protocol |
N/A
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Description |
Gene Expression CodeSet profiling 254 genes; 248 inflammation-related mouse genes and 6 housekeeping genes.
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Data processing |
Data was normalized, quantified, and analyzed using the nSolver software (Nanostring Technologies Inc., Seattle, WA) with default parameters to noramlize data to housekeeping genes.
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Submission date |
Apr 18, 2022 |
Last update date |
Aug 10, 2022 |
Contact name |
Andrew Oberst |
E-mail(s) |
oberst@uw.edu
|
Organization name |
University of Washington
|
Street address |
750 Republican street
|
City |
Seattle |
State/province |
WA |
ZIP/Postal code |
98109 |
Country |
USA |
|
|
Platform ID |
GPL20885 |
Series (1) |
GSE200985 |
ADAR1 mutation causes ZBP1-dependent immunopathology I |
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