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Sample GSM6047847 Query DataSets for GSM6047847
Status Public on Aug 10, 2022
Title 20210827_AOberst-20210826-01_7_07
Sample type RNA
 
Source name mouse embryonic fibroblasts
Organism Mus musculus
Characteristics cell type: mouse embryonic fibroblasts
genotype: MLKLko
sirna: siADAR
Treatment protocol 5x10^5 cells were transfected with siRNA using Dharmafect reagent for 24 hours.
Growth protocol Cell were grown in complete DMEM medium in 12 well plates.
Extracted molecule total RNA
Extraction protocol Cell were collected in TRIzol reageatnt and total RNA was extracted by phenol-chloroform phase separation followed by RNA precipitation according to the manufacturer's protocol.
Label Capture probe-3'biotin, Reporter probe-5' barcode signal
Label protocol NanoString nCounter XT gene expression assay protocol
 
Hybridization protocol NanoString nCounter XT gene expression assay protocol
Scan protocol N/A
Description Gene Expression CodeSet profiling 254 genes; 248 inflammation-related mouse genes and 6 housekeeping genes.
Data processing Data was normalized, quantified, and analyzed using the nSolver software (Nanostring Technologies Inc., Seattle, WA) with default parameters to noramlize data to housekeeping genes.
 
Submission date Apr 18, 2022
Last update date Aug 10, 2022
Contact name Andrew Oberst
E-mail(s) oberst@uw.edu
Organization name University of Washington
Street address 750 Republican street
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL20885
Series (1)
GSE200985 ADAR1 mutation causes ZBP1-dependent immunopathology I

Data table header descriptions
ID_REF
VALUE normalized signal

Data table
ID_REF VALUE
Ager 4.872819582
Alox12 2.785356741
Alox15 3.955281742
Alox5 4.244788359
Areg 6.914639757
Arg1 3.370319241
Atf2 10.09369577
Bcl2l1 9.970232083
Bcl6 5.82975086
Birc2 8.704219978
C1qa 3.785356741
C1qb 3.107284835
C1ra 7.070758959
C2 3.370319241
C3 4.244788359
C3ar1 5.592711663
C4a 5.955281742
C6 4.592711663
C7 4.485796459
C8a 3.955281742

Total number of rows: 248

Table truncated, full table size 4 Kbytes.




Supplementary file Size Download File type/resource
GSM6047847_20210827_AOberst-20210826-01_7_07.RCC.gz 3.1 Kb (ftp)(http) RCC
Processed data included within Sample table

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