|
| Status |
Public on Aug 10, 2022 |
| Title |
20210827_AOberst-20210826-01_12_12 |
| Sample type |
RNA |
| |
|
| Source name |
mouse embryonic fibroblasts
|
| Organism |
Mus musculus |
| Characteristics |
cell type: mouse embryonic fibroblasts
genotype: CASP8ko_MLKLko
sirna: siADAR
|
| Treatment protocol |
5x10^5 cells were transfected with siRNA using Dharmafect reagent for 24 hours.
|
| Growth protocol |
Cell were grown in complete DMEM medium in 12 well plates.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cell were collected in TRIzol reageatnt and total RNA was extracted by phenol-chloroform phase separation followed by RNA precipitation according to the manufacturer's protocol.
|
| Label |
Capture probe-3'biotin, Reporter probe-5' barcode signal
|
| Label protocol |
NanoString nCounter XT gene expression assay protocol
|
| |
|
| Hybridization protocol |
NanoString nCounter XT gene expression assay protocol
|
| Scan protocol |
N/A
|
| Description |
Gene Expression CodeSet profiling 254 genes; 248 inflammation-related mouse genes and 6 housekeeping genes.
|
| Data processing |
Data was normalized, quantified, and analyzed using the nSolver software (Nanostring Technologies Inc., Seattle, WA) with default parameters to noramlize data to housekeeping genes.
|
| |
|
| Submission date |
Apr 18, 2022 |
| Last update date |
Aug 10, 2022 |
| Contact name |
Andrew Oberst |
| E-mail(s) |
oberst@uw.edu
|
| Organization name |
University of Washington
|
| Street address |
750 Republican street
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL20885 |
| Series (1) |
| GSE200985 |
ADAR1 mutation causes ZBP1-dependent immunopathology I |
|
