|
| Status |
Public on Jun 27, 2022 |
| Title |
ZDF 4 |
| Sample type |
SRA |
| |
|
| Source name |
serum
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: serum
treatment: PTH
group: ZDF
disease state: model of type II diabetes
time: samples collected 12 weeks after receiving treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was performed in 100 microL of serum samples and ulna bone homogenized samples with miRNeasy Mini Kits (Qiagen)
RealSeq-Biofluids Plasma/Serum miRNA Library kit for Illumina sequencing
|
| |
|
| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
NextSeq 550 |
| |
|
| Data processing |
fastQ quality assessment
cutadapt adater trimming
bowtie1 genome alignment
miRDeep2 miRNA quantification
edgeR differential expression
Assembly: Rnor.6.0 (GCA_000001895.4)
Supplementary files format and content: CSV with miRNA quantifications (raw and rpm)
|
| |
|
| Submission date |
Apr 21, 2022 |
| Last update date |
Jun 27, 2022 |
| Contact name |
Andreas B Diendorfer |
| E-mail(s) |
service@tamirna.com
|
| Organization name |
TAmiRNA GmbH
|
| Street address |
Leberstraße 20
|
| City |
Wien |
| ZIP/Postal code |
1110 |
| Country |
Austria |
| |
|
| Platform ID |
GPL25029 |
| Series (1) |
| GSE201231 |
Effect of anti-osteoporotic treatments on circulating and bone microRNA patterns in osteopenic ZDF ratsEffect of anti-osteoporotic treatments on circulating and bone microRNA patterns in osteopenic ZDF rats |
|
| Relations |
| BioSample |
SAMN27723700 |
| SRA |
SRX14953936 |
