|
| Status |
Public on Jan 04, 2023 |
| Title |
Control cortex Replicate 3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
IP cortex,normal
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: cortex
disease state: normal
gender: male
|
| Treatment protocol |
After harvesting on ice, snap frozen in liquid nitrogen, and stored at - 80 ° C
|
| Growth protocol |
Drink and eat freely
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
"IP" and "Sup" RNAs were amplified as cRNAs and labeled with Cy5 and Cy3 respectively using Arraystar Super RNA Labeling Kit.
|
| |
|
| Channel 2 |
| Source name |
Sup cortex,normal
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: cortex
disease state: normal
gender: male
|
| Treatment protocol |
After harvesting on ice, snap frozen in liquid nitrogen, and stored at - 80 ° C
|
| Growth protocol |
Drink and eat freely
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
"IP" and "Sup" RNAs were amplified as cRNAs and labeled with Cy5 and Cy3 respectively using Arraystar Super RNA Labeling Kit.
|
| |
|
| |
| Hybridization protocol |
Cy3 and Cy5 labeled cRNAs were combined together and hybridized to Arraystar Rat mRNA&lncRNA Epitranscriptomic Arrays (8x60K, Arraystar) at 65°C for 17 hours in an Agilent Hybridization Oven.
|
| Scan protocol |
After washing, slides were scanned with Agilent Scanner G2505C.
|
| Description |
Biological replicate 3 of 3. Control rat cortex.
A3
|
| Data processing |
Data was extracted using Agilent Feature Extraction software. The probe signals passing "P" or "M" QC flags in a certain proportion were retained for further analysis.
processed data includes normalized signal for IP and Sup.
|
| |
|
| Submission date |
Apr 21, 2022 |
| Last update date |
Jan 04, 2023 |
| Contact name |
Bo-wei CHEN |
| E-mail(s) |
201207020217@stu.hnucm.edu.cn
|
| Phone |
86-0731-85366925
|
| Organization name |
Hunan University of Chinese Medicine
|
| Street address |
No. 95 Shaoshan Middle Road
|
| City |
Changsha |
| ZIP/Postal code |
410007 |
| Country |
China |
| |
|
| Platform ID |
GPL25916 |
| Series (1) |
| GSE201258 |
Genome-wide identification of m6A-related transcriptional profiles in cortex of rats with cerebral ischemia-reperfusion and intervention of Buyang Huanwu Decoction |
|
