|
Status |
Public on Jan 04, 2023 |
Title |
model cortex after BHD intervention Replicate 2 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
IP cortex,BHD intervention 7days
|
Organism |
Rattus norvegicus |
Characteristics |
tissue: cortex disease state: cerebral ischemia-reperfusion injury with BHD gender: male
|
Treatment protocol |
After harvesting on ice, snap frozen in liquid nitrogen, and stored at - 80 ° C
|
Growth protocol |
Drink and eat freely
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
Label |
Cy5
|
Label protocol |
"IP" and "Sup" RNAs were amplified as cRNAs and labeled with Cy5 and Cy3 respectively using Arraystar Super RNA Labeling Kit.
|
|
|
Channel 2 |
Source name |
Sup cortex,BHD intervention 7days
|
Organism |
Rattus norvegicus |
Characteristics |
tissue: cortex disease state: cerebral ischemia-reperfusion injury with BHD gender: male
|
Treatment protocol |
After harvesting on ice, snap frozen in liquid nitrogen, and stored at - 80 ° C
|
Growth protocol |
Drink and eat freely
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
Label |
Cy3
|
Label protocol |
"IP" and "Sup" RNAs were amplified as cRNAs and labeled with Cy5 and Cy3 respectively using Arraystar Super RNA Labeling Kit.
|
|
|
|
Hybridization protocol |
Cy3 and Cy5 labeled cRNAs were combined together and hybridized to Arraystar Rat mRNA&lncRNA Epitranscriptomic Arrays (8x60K, Arraystar) at 65°C for 17 hours in an Agilent Hybridization Oven.
|
Scan protocol |
After washing, slides were scanned with Agilent Scanner G2505C.
|
Description |
Biological replicate 2 of 3. erebral ischemia-reperfusion model rat cortex after BHD intervention.7days. C2
|
Data processing |
Data was extracted using Agilent Feature Extraction software. The probe signals passing "P" or "M" QC flags in a certain proportion were retained for further analysis. processed data includes normalized signal for IP and Sup.
|
|
|
Submission date |
Apr 21, 2022 |
Last update date |
Jan 04, 2023 |
Contact name |
Bo-wei CHEN |
E-mail(s) |
201207020217@stu.hnucm.edu.cn
|
Phone |
86-0731-85366925
|
Organization name |
Hunan University of Chinese Medicine
|
Street address |
No. 95 Shaoshan Middle Road
|
City |
Changsha |
ZIP/Postal code |
410007 |
Country |
China |
|
|
Platform ID |
GPL25916 |
Series (1) |
GSE201258 |
Genome-wide identification of m6A-related transcriptional profiles in cortex of rats with cerebral ischemia-reperfusion and intervention of Buyang Huanwu Decoction |
|