|
| Status |
Public on Oct 15, 2010 |
| Title |
whole lungs (pooled in triplicate) mock treated 24hpi |
| Sample type |
RNA |
| |
|
| Source name |
whole lung
|
| Organism |
Mus musculus |
| Characteristics |
tissue: whole lung
strain: B6.A2G-Mx1-FNAR1-/-IL28R{alpha}-/-
treatment group: mock treated, 24hpi
|
| Treatment protocol |
See Schmid et. Al. JBC 2010
|
| Growth protocol |
See Schmid et. Al. JBC 2010
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
Cy3
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Hybridization Oven 640.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Array Scanner 7GGeneChips were scanned using the GeneChip Array Scanner 7G
|
| Data processing |
The data were analyzed with GeneChip Operating Software (GCOS) ver 1.3
MAS5-calculated Signal Intensity
|
| |
|
| Submission date |
Oct 14, 2010 |
| Last update date |
Oct 14, 2010 |
| Contact name |
Benjamin tenOever |
| Organization name |
New York University
|
| Department |
Microbiology
|
| Lab |
tenOever lab
|
| Street address |
430 East 29th Street, Room 540
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10016 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE24695 |
Transcription Factor Redundancy Ensures Induction of the Antiviral State |
|
