|
| Status |
Public on Oct 15, 2010 |
| Title |
Transcription Factor Redundancy Ensures Induction of the Antiviral State |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Transcriptional response to virus infection in mice lacking type I and type III signaling
The transcriptional response to virus infection is thought to be predominantly induced by interferon (IFN) signaling. Here we demonstrate that, in the absence of IFN signaling, an IFN-like transcriptome is still maintained. This transcriptional activity is mediated from IFN-stimulated response elements (ISREs) that bind to both the IFN stimulated gene factor 3 (ISGF3) as well as to IFN response factor 7 (IRF7). Through a combination of both in vitro biochemistry and in vivo transcriptional profiling, we have dissected what constitutes IRF-specific, ISGF3-specific, or universal ISREs. Taken together, the data presented here suggests that IRF7 can induce an IFN-like transcriptome in the absence of type-I or -III signaling and therefore provides a level of redundancy to cells to ensure the induction of the antiviral state.
|
| |
|
| Overall design |
Mouse: Infections were performed in triplicate and lungs from each condition were pooled for total RNA isolation.
Human: Analysis of IRF7 transcriptome in U3A cell line was performed in duplicates.
|
| |
|
| Contributor(s) |
Schmid S, tenOever BR |
| Citation(s) |
20943654 |
| Submission date |
Oct 14, 2010 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Benjamin tenOever |
| Organization name |
New York University
|
| Department |
Microbiology
|
| Lab |
tenOever lab
|
| Street address |
430 East 29th Street, Room 540
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10016 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (13)
|
| GSM608527 |
whole lungs (pooled in triplicate) mock treated 12hpi |
| GSM608528 |
whole lungs (pooled in triplicate) PR/8/34/A treated 12hpi |
| GSM608529 |
whole lungs (pooled in triplicate) PR/8/34/AdeltaNS1 treated 12hpi |
| GSM608530 |
whole lungs (pooled in triplicate) mock treated 24hpi |
| GSM608531 |
whole lungs (pooled in triplicate) PR/8/34/A treated 24hpi |
| GSM608532 |
whole lungs (pooled in triplicate) PR/8/34/AdeltaNS1 treated 24hpi |
| GSM608533 |
whole lungs (pooled in triplicate) mock treated 48hpi |
| GSM608534 |
whole lungs (pooled in triplicate) PR/8/34/A treated 48hpi |
| GSM608535 |
whole lungs (pooled in triplicate) PR/8/34/AdeltaNS1 treated 48hpi |
| GSM609460 |
U3A_GFP_20h_rep1 |
| GSM609461 |
U3A_GFP_20h_rep2 |
| GSM609462 |
U3A_IRF7+IKKe_20h_rep1 |
| GSM609463 |
U3A_IRF7+IKKe_20h_rep2 |
|
| Relations |
| BioProject |
PRJNA132269 |
Less...
More...