|
| Status |
Public on Oct 18, 2010 |
| Title |
U3A_IRF7+IKKe_20h_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
U3A cells, treated with type I IFN and transfected with IRF7 and IKKe, 20h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: U3A cells (fibrosarcoma), deficient in STAT1
treatment: 25 IU/ml type I IFN
transfection: IRF7 + IKKe
time post transfection: 20h
|
| Treatment protocol |
See Schmid et. Al. JBC 2010
|
| Growth protocol |
See Schmid et. Al. JBC 2010
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
Cy3
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Hybridization Oven 640.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Array Scanner 7GGeneChips were scanned using the GeneChip Array Scanner 7G
|
| Description |
IRF7/IKKe_rep1
|
| Data processing |
The data were analyzed with GeneChip Operating Software (GCOS) ver 1.3
|
| |
|
| Submission date |
Oct 18, 2010 |
| Last update date |
Oct 18, 2010 |
| Contact name |
Benjamin tenOever |
| Organization name |
New York University
|
| Department |
Microbiology
|
| Lab |
tenOever lab
|
| Street address |
430 East 29th Street, Room 540
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10016 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE24695 |
Transcription Factor Redundancy Ensures Induction of the Antiviral State |
|
