|
| Status |
Public on Oct 21, 2010 |
| Title |
Global gene expression of NASH patient, P34 |
| Sample type |
RNA |
| |
|
| Source name |
Patient liver biopsy
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: non-alcoholic steatohepatitis (NASH)
tissue: liver
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Samples were stored in RNAlater at -80 freezer before total RNA extraction with Qiagen RNeasy kit.
|
| Label |
biotin
|
| Label protocol |
Complementary DNA was prepared from total RNA.
In vitro transcription was then performed with biotin labeled substrate to produce biotin labeled cRNA.
|
| |
|
| Hybridization protocol |
1, Fragmentation of cRNA.
2, Denature cRNA at 90C for 5 min.
3, Hybridization was performed at 37 C, for 18-24 hours.
4, Detection with Cy5-streptavidin conjugate.
|
| Scan protocol |
Microarray scanning and data acquisition were done at the gene expression facility, Roswell Park Cancer Institute, following their routine standard procedure.
|
| Data processing |
Data were median-normalized for comparison. Medium normalization were done by our collaborators at Roswell Park cancer institute and verified using MS Excel2003.
|
| |
|
| Submission date |
Oct 19, 2010 |
| Last update date |
Oct 20, 2010 |
| Contact name |
Susan Baker |
| E-mail(s) |
sbaker@upa.chob.edu, lixinzhu@buffalo.edu
|
| Organization name |
SUNY at Buffalo
|
| Department |
Pediatrics
|
| Lab |
Digestive Diseases and Nutrition Center Research Laboratory
|
| Street address |
3435 Main street, 422 BRB
|
| City |
Buffalo |
| State/province |
NY |
| ZIP/Postal code |
14214 |
| Country |
USA |
| |
|
| Platform ID |
GPL2895 |
| Series (1) |
| GSE24807 |
Increased hemoglobin expression in NASH livers |
|
