|
| Status |
Public on Mar 13, 2023 |
| Title |
F0(Express617 x G3D001),15 days ovule,rep1 [F0_Rep1_E15] |
| Sample type |
SRA |
| |
|
| Source name |
ovule
|
| Organism |
Brassica napus |
| Characteristics |
tissue: ovule
genotype: F0(Express617 x G3D001)
time: 15 days after ovule fertilization
|
| Growth protocol |
Express617, G3D001 and its F1 plants were planted in the natural environment of the potting field in Huazhong Agricultural University
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Small RNA was extracted using Plant miRNA kit . 1 ug of total RNA was used for the construction of sequencing libraries.
sRNA libraries for miRNA-seq were prepared using NEB Next Multiplex Small RNA Library Prep Set for Illumina following manufacturer's protocols.
|
| |
|
| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Filter reads shorter than 18nt using seqtk 1.3
Sequence reads were mapped to Express617 and were quantified using ShortStack 3.8.5
Assembly: Express617_v1
Supplementary files format and content: Counts.txt
|
| |
|
| Submission date |
May 10, 2022 |
| Last update date |
Mar 13, 2023 |
| Contact name |
Hao Wang |
| E-mail(s) |
haowang@webmail.hzau.edu.cn
|
| Phone |
18715003498
|
| Organization name |
Huazhong Agricultural University
|
| Department |
National Key Laboratory of Crop Genetic Improvement
|
| Street address |
No. 1, Shizishan Street, Hongshan District
|
| City |
Wuhan |
| ZIP/Postal code |
430070 |
| Country |
China |
| |
|
| Platform ID |
GPL29911 |
| Series (2) |
| GSE202608 |
Transgressive and parental dominant gene expression and cytosine methylation during seed development in Brassica napus hybrids [miRNA-seq] |
| GSE202610 |
Transgressive and parental dominant gene expression and cytosine methylation during seed development in Brassica napus hybrids |
|
| Relations |
| BioSample |
SAMN28173612 |
| SRA |
SRX15220716 |
