|
| Status |
Public on Dec 24, 2010 |
| Title |
L4_wild-type_run1 |
| Sample type |
SRA |
| |
|
| Source name |
whole animal
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
strain: Bristol N2
developmental stage: L4
|
| Growth protocol |
Nematodes were grown under standard conditions
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
3P-Seq; see http://web.wi.mit.edu/bartel/pub/protocols.html
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Data processing |
Reads were reverse complemented and aligned to the C. elegans genome (ce6/ws190) using Bowtie. Reads that aligned best to one genomic locus and had one or more mismatches at their 3' end within a terminal adenylate-run were carried forward as 3P tags. Reads mapping to the same locus with the same number of terminal adenylates were consolidated in the processed data file.
|
| |
|
| Submission date |
Oct 26, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
calvin jan |
| E-mail(s) |
cjan@wi.mit.edu
|
| Organization name |
WIBR/MIT/HHMI
|
| Street address |
9 Cambridge center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platform ID |
GPL9269 |
| Series (1) |
| GSE24924 |
Formation, regulation and evolution of 3' UTRs in Caenorhabditis elegans |
|
| Relations |
| SRA |
SRX029178 |
| BioSample |
SAMN00116620 |
