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Sample GSM614246 Query DataSets for GSM614246
Status Public on Feb 01, 2014
Title Transformant yeast 1 M sorbitol 60 min rep2
Sample type RNA
 
Source name S. cerevisiae expressing C. albicans Nik1p, sorbitol, 60 min
Organism Saccharomyces cerevisiae
Characteristics strain: BWG1-7a
expression vector: pYES2+CaNIK1
treatment: 1 M sorbitol
duration of treatment: 60 min
Treatment protocol Induction of transgene expression was achieved by incubation of transformants in SG-ura at 30°C for 16h, and functional analysis of the recombinant strain was carried out in SG-ura.
Cultures were treated with 1 M sorbitol.
Growth protocol S. cerevisiae transformants were maintained in SD-ura (0.67% yeast nitrogen base without amino acids, 2% glucose, nucleosides and amino acids according to Rose, M. D., Winston, F., and Hieter, P. (1990) Methods in Yeast Genetics: A Laboratory Course Manual, Revised Ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, without uracil) at 30°C.
Extracted molecule total RNA
Extraction protocol Cells were disrupted using glass beads (425-600 µm, Sigma) in the presence of lysis buffer (Qiagen RLT). RNA was isolated using RNeasy columns (Qiagen) according to the manufacturer's protocol.
Label biotin
Label protocol 500 ng of total RNA were used for biotin labeling according to the 3´ IVT Express Kit (Affymetrix).
 
Hybridization protocol Samples were hybridized to an identical lot of Affymetrix GeneChip Yeast Genome 2.0 Arrays for 16 hours at 45°C. Steps for washing and SA-PE staining were processed on the fluidics station 450 using the mini_euk2v3_FS450 protocol (Affymetrix).
Scan protocol Image analysis was performed using a GCS3000 Scanner and GCOS1.2 Software Suite (Affymetrix).
Description O60.2
Data processing Raw intensities were RMA (robust multi-array average) normalized using the affy package of Bioconductor/R.
 
Submission date Oct 28, 2010
Last update date Jan 23, 2015
Contact name Anna Buschart
E-mail(s) anna.buschart@helmholtz-hzi.de
Organization name Helmholtz-Centre for Infection Research
Department BiSA
Street address Inhoffenstr. 7
City Braunschweig
ZIP/Postal code 38124
Country Germany
 
Platform ID GPL2529
Series (1)
GSE25002 Expression data from Saccharomyces cerevisiae expressing Candida albicans Nik1p comparing osmotic stress and fludioxonil treatment

Data table header descriptions
ID_REF
VALUE Log2 RMA-normalized value

Data table
ID_REF VALUE
1769308_at 10.28094039
1769311_at 12.49739551
1769312_at 9.651771253
1769313_at 9.019554805
1769314_at 11.61755622
1769317_at 10.54965694
1769319_at 11.90816962
1769320_at 8.404745231
1769321_at 11.01615671
1769322_s_at 8.819707808
1769323_at 10.41610798
1769324_at 10.11396977
1769325_at 8.345385599
1769329_at 10.84413842
1769331_at 11.86583718
1769333_at 11.97337401
1769335_at 11.71322722
1769336_at 9.547796004
1769338_at 8.360660583
1769339_at 10.83533409

Total number of rows: 5900

Table truncated, full table size 133 Kbytes.




Supplementary file Size Download File type/resource
GSM614246.CEL.gz 984.8 Kb (ftp)(http) CEL
Processed data included within Sample table

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