|
| Status |
Public on Jul 29, 2022 |
| Title |
motor_neuron_E33_EMR_backfill |
| Sample type |
SRA |
| |
|
| Source name |
spinal cord
|
| Organism |
Gallus gallus |
| Characteristics |
celltype: motor neuron
tissue: spinal cord
age: embryonic day 9
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Backfilled spinal cord was dissociated with Papain (0,25 mg/ml) and individual fluorescent cells were transferred to PCR strips.
SMART-seq2
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
E33
counts_MN_smartseq2.csv.gz
|
| Data processing |
Raw reads were trimmed with Trimmomatic (v.0.39).
Trimmed reads were aligned with STAR (v.2.5.2) to the GRCg6a chicken genome.
Count tables were created with HTSeq (v.0.6.1).
Assembly: GRCg6a
Supplementary files format and content: Comma separated values file (.csv) . Count table created by HTSeq where genes are rows and cells are columns.
|
| |
|
| Submission date |
May 23, 2022 |
| Last update date |
Jul 29, 2022 |
| Contact name |
Fabio Sacher |
| E-mail(s) |
fabio.sacher@bluewin.ch
|
| Organization name |
Universität Basel
|
| Department |
Umweltwissenschaften
|
| Lab |
Regulatory Evolution
|
| Street address |
Vesalgasse 1
|
| City |
Basel |
| State/province |
Basel-Stadt |
| ZIP/Postal code |
4051 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL26853 |
| Series (1) |
| GSE203595 |
A method to investigate muscle target-specific transcriptional signatures of single motor neurons |
|
| Relations |
| BioSample |
SAMN28597602 |
| SRA |
SRX15414642 |
