|
| Status |
Public on Dec 20, 2010 |
| Title |
HoxA3-3 |
| Sample type |
RNA |
| |
|
| Source name |
Endothelial cells, co-cultured on OP9, 5 days,Doxycycline treatment,control transduced
|
| Organism |
Mus musculus |
| Characteristics |
strain: 129P2 (also referred to as 129/Ola)
cell type: EBs derived endothelial progenitors
transduction protocol: HoxA3 upregulation
doxycycline: yes
|
| Treatment protocol |
Samples were transduced with the appropriate virus and treated with Doxycycline for 5 days, except for the control.
|
| Growth protocol |
endothelial cells derived from day 6 Ebs cultured on OP9 stroma for 5 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
5000 cells were sorted into SuperAmp Lysis Buffer and shipped to Miltenyi.
|
| Label |
Cy3
|
| Label protocol |
250 ng of each cDNA were used as template for Cy3 labeling which was performed by Miltenyi.
|
| |
|
| Hybridization protocol |
Cy3-labeled cDNAs were hybridized overnight (17 hours, 65 C) to Agilent Whole Mouse Genome Oligo Microarrays 4 x 44K.
|
| Scan protocol |
Fluorescence signals were detected using Agilent's Microarray Scanner System
|
| Description |
251486828226_S01_GE1_105_Jan09_1_2.txt
|
| Data processing |
Raw files were analyzed in genespring11.2 by using the following parameters: Normalization - shift to 75 percentile, Baseline transformation - median of all samples
|
| |
|
| Submission date |
Nov 04, 2010 |
| Last update date |
Dec 20, 2010 |
| Contact name |
Michael Kyba |
| Organization name |
University of Minnesota
|
| Lab |
Kyba Lab
|
| Street address |
312 Church St. SE
|
| City |
Minneapolis |
| State/province |
MN |
| ZIP/Postal code |
55455 |
| Country |
USA |
| |
|
| Platform ID |
GPL4134 |
| Series (2) |
| GSE25079 |
Epistasis analysis of Runx1 and Gata1 over HoxA3 in hemogenic endothelium |
| GSE25096 |
HoxA3 is an apical regulator of hemogenic endothelium |
|
