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Sample GSM61902

Query DataSets for GSM61902

Status

Public on Jun 22, 2005

Title

Giessen-WT_B

Sample type

RNA

Source name

Rhodobacter sphaeroides

Organism

Cereibacter sphaeroides

Characteristics

n/a

Growth protocol

The cells were cultivated at 32 °C in malate minimal salt medium under semiaerobic conditions (approx. 0.85 mg l-1 dissolved oxygen) with continuous shaking at 140 rpm.

Extracted molecule

total RNA

Extraction protocol

Cells (at OD600~0.5) were collected by centrifugation. Total RNA was isolated by the hot phenol method, quantified by spectrophotometric analysis (A260).

Label

n/a

Description

control culture for oxidative stress experiments

Data processing

MAS 5.0 (CEL files for alternative processing are available)

Submission date

Jun 20, 2005

Last update date

Jun 21, 2005

Contact name

Mark Gomelsky

E-mail(s)

gomelsky@uwyo.edu

Phone

307-766-3522

Organization name

University of Wyoming

Department

Molecular Biology

Lab

Gomelsky

Street address

City

Laramie

State/province

ZIP/Postal code

82071

Country

USA

Platform ID

GPL162

Series (1)

GSE2829

Regulation of hydrogen peroxide-dependent gene expression in Rhodobacter sphaeroides: Regulatory functions of OxyR

Data table header descriptions
ID_REF
VALUE	relative signal of expression
ABS_CALL	Present if p<0.04 Absent if p>0.06 Marginal if 0.04 < p < 0.06
DETECTION P-VALUE	stat. significance of detection
Stat Pairs	pairs of PM-MM present on chip
Stat Pairs Used	pairs of PM-MM used in analysis

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE	Stat Pairs	Stat Pairs Used
1	16.2	A	0.537511	14	14
2	46.2	A	0.150145	14	14
3	3.8	A	0.914381	20	19
4	16.5	A	0.116113	20	20
5	23.7	P	0.033677	20	20
6	2.6	A	0.921522	20	18
7	18	A	0.089478	20	20
8	19.4	A	0.227617	20	20
9	33.9	A	0.153051	20	18
10	10.3	A	0.48511	20	20
11	2.3	A	0.574038	20	20
12	2.1	A	0.883887	20	20
13	3.9	A	0.51489	20	20
14	0.6	A	0.977896	20	19
15	0.2	A	0.891021	20	20
16	0.1	A	0.389089	20	19
17	14.2	P	0.039661	20	20
18	3.8	A	0.846949	20	18
19	1.4	A	0.950521	20	19
20	0.6	A	0.814869	20	20

Total number of rows: 5355

Table truncated, full table size 143 Kbytes.

Supplementary data files not provided