|
| Status |
Public on Jun 22, 2005 |
| Title |
Giessen_dOxyR_7C |
| Sample type |
RNA |
| |
|
| Source name |
Rhodobacter sphaeroides
|
| Organism |
Cereibacter sphaeroides |
| Characteristics |
n/a
|
| Treatment protocol |
1 mM H2O2 (final concentration) for 7 min
|
| Growth protocol |
The cells were cultivated at 32 °C in malate minimal salt medium under semiaerobic conditions (approx. 0.85 mg l-1 dissolved oxygen) with continuous shaking at 140 rpm.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells (at OD600~0.5) were collected by centrifugation. Total RNA was isolated by the hot phenol method, quantified by spectrophotometric analysis (A260).
|
| Label |
n/a
|
| |
|
| Description |
hydrogen peroxide stress in delta-oxyR mutant; 7 min after H2O2 addition
|
| Data processing |
MAS 5.0 (CEL files for alternative processing are available)
|
| |
|
| Submission date |
Jun 20, 2005 |
| Last update date |
Jun 21, 2005 |
| Contact name |
Mark Gomelsky |
| E-mail(s) |
gomelsky@uwyo.edu
|
| Phone |
307-766-3522
|
| Organization name |
University of Wyoming
|
| Department |
Molecular Biology
|
| Lab |
Gomelsky
|
| Street address |
|
| City |
Laramie |
| State/province |
WY |
| ZIP/Postal code |
82071 |
| Country |
USA |
| |
|
| Platform ID |
GPL162 |
| Series (1) |
| GSE2829 |
Regulation of hydrogen peroxide-dependent gene expression in Rhodobacter sphaeroides: Regulatory functions of OxyR |
|
