|
| Status |
Public on Nov 13, 2023 |
| Title |
WT_Gen4_Line2_sib |
| Sample type |
SRA |
| |
|
| Source name |
leaf tissue
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: leaf tissue
generation: 4
genotype: WT
lineage: 2
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA (gDNA) was extracted from 1-month-old Arabidopsis thaliana rosette leaves with the DNeasy plant mini kit (Qiagen, cat. no. 69104) per the manufacturer’s instructions.
Libraries were produced with the Ultra II DNA Library prep kit according to manufacturer’s instructions (New England Biolabs, cat. no. E7645L). Bisulfite conversion of DNA was carried out according to manufacturer’s protocol (Zymo, EZ DNA Methylation Lightning Kit, cat. no. D5046). DNA was converted twice to ensure complete bisulfite conversion of unmethylated cytosine.
|
| |
|
| Library strategy |
Bisulfite-Seq |
| Library source |
genomic |
| Library selection |
RANDOM |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Assembly: Araport 11
GFF files containing C/T counts for cytosines in the CG context
|
| |
|
| Submission date |
May 25, 2022 |
| Last update date |
Nov 13, 2023 |
| Contact name |
Daniel Zilberman |
| E-mail(s) |
daniel.zilberman@ista.ac.at
|
| Organization name |
Institute of Science and Technology Austria
|
| Street address |
Am Campus 1
|
| City |
Klosterneuburg |
| ZIP/Postal code |
3040 |
| Country |
Austria |
| |
|
| Platform ID |
GPL19580 |
| Series (1) |
| GSE204837 |
A quantitative model of MET1-catalyzed establishment and epigenetic inheritance of DNA methylation |
|
| Relations |
| BioSample |
SAMN28671309 |
| SRA |
SRX15467271 |
