|
| Status |
Public on Nov 11, 2010 |
| Title |
Bisulfite-Seq analysis of RRBS533 derived from human CD34 cells; 42U16AAXX100205-7-S |
| Sample type |
SRA |
| |
|
| Source name |
flow sorted CD34+ cells from cord blood; 42U16AAXX100205-7-S
|
| Organism |
Homo sapiens |
| Characteristics |
molecule: genomic DNA
disease: none
biomaterial_provider: FHCRC
biomaterial_type: Primary Cell
cell_type: CD34 Primary Cells
markers: CD34+
donor_id: CD34_0/0/00
donor_age: NA
donor_health_status: none
donor_sex: Unknown
donor_ethnicity: NA
passage_if_expanded: not passaged
experiment_type: DNA Methylation
extraction_protocol: Standard Protocol (Smith et al., Methods 48, 226-232)
extraction_protocol_type_of_sonicator: NA
extraction_protocol_sonication_cycles: NA
dna_preparation_initial_dna_qnty: 20 ng
dna_preparation_fragment_size_range: 40-160 or 90-260
dna_preparation_adaptor_sequence: 5' P-GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTG and 5' ACACTCTTTCCCTACACGACGCTCTTCCGATCT
dna_preparation_adaptor_ligation_protocol: T4 Ligase (2,000U/ul) 16C o/n
dna_preparation_post-ligation_fragment_size_selection: 180-350 or 130-250
bisulfite_conversion_protocol: 2x5hrs Epitect Kit
bisulfite_conversion_percent: >99%
library_generation_pcr_template_conc: NA
library_generation_pcr_polymerase_type: Pfu Turbo Cx hot start
library_generation_pcr_thermocycling_program: Smith et al., Methods 48, 226-232
library_generation_pcr_number_cycles: 15.0
library_generation_pcr_f_primer_sequence: 5' AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
library_generation_pcr_r_primer_sequence: 5' CAAGCAGAAGACGGCATACGAGCTCTTCCGATCT
library_generation_pcr_primer_conc: 25uM
library_generation_pcr_product_isolation_protocol: Gel size selection
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Library construction protocol: Single read - Illumina
|
| |
|
| Library strategy |
Bisulfite-Seq |
| Library source |
genomic |
| Library selection |
Reduced Representation |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
sample_term_id: CL_0000837
assay_term_id: OBI_0001862
nucleic_acid_term_id: SO_0000352
Design description: RRBS REMC Sequencing on Illumina
Library name: Solexa-17943/Solexa-17919
EDACC Genboree Experiment Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FEXPERIMENT%2FEDACC.2318
EDACC Genboree Sample Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FSAMPLE%2FEDACC.2302
****************
For data usage terms and conditions, please refer to:
http://www.drugabuse.gov/funding/funding-opportunities/nih-common-fund/epigenomics-data-access-policies
****************
|
| Data processing |
**********************************************************************
ANALYSIS FILE NAME: GSM621559_BI.CD34_Primary_Cells.RRBS.CD34_0_0_00.RRBS532-533.wig
ANALYSIS CENTER: EDACC
ANALYSIS ALIAS: 42U16AAXX100205-7-S-42TY1AAXX100126-2-S.hg19.level.2
ANALYSIS TITLE: Methylation Proportion Graphs of CD34 Primary Cells RRBS Data
ANALYSIS DESCRIPTION: Illumina RRBS read mappings from CD34 Primary Cells, Donor CD34_0/0/00, Libraries RRBS532-533 were processed into graphs of methylation proportions. Methylation proportions were calculated as (methylated calls / (methylated calls + unmethylated calls)) for all CpGs covered by at least 4 reads.
ANALYSIS TYPE: ABUNDANCE_MEASUREMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.5216
DATA_ANALYSIS_LEVEL: 2
EXPERIMENT_TYPE: Reduced Representation Bisulfite-Seq
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19 MspI restriction fragments 40-220bp
SOFTWARE: In house programs and scripts
SOFTWARE_VERSION: NA
READ_EXTENSION: 0bp
TREATMENT_OF_IDENTICAL_ALIGNMENTS_OF_MULTIPLE_READS: None
GENOMIC_WINDOW: 2bp containing CpGs
TREATMENT_OF_REGIONS_PRONE_TO_MULTIPLE_ALIGNMENTS: None
RELEASE_NUMBER: Human Epigenome Atlas 2
BROWSER_TRACK_NAME: CD34 RRBS 32
BROWSER_TRACK_DESCRIPTION: BI CD34 Primary Cells RRBS Donor CD34_0/0/00 Libraries RRBS532-533 EA Release 2
BISULFITE_CONVERSION_PERCENTAGE_BASED_ON_MAPPINGS: 99.28
**********************************************************************
|
| |
|
| Submission date |
Nov 10, 2010 |
| Last update date |
Jan 29, 2015 |
| Contact name |
BROAD INSTITUTE |
| E-mail(s) |
rharris1@bcm.tmc.edu
|
| Organization name |
Broad Institute
|
| Street address |
-
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL9115 |
| Series (1) |
| GSE25247 |
BI Human Reference Epigenome Mapping Project: Characterization of DNA methylation by RRBS in human subject |
|
| Relations |
| BioSample |
SAMN00012124 |
| Named Annotation |
GSM621559_BI.CD34_Primary_Cells.RRBS.CD34_0_0_00.RRBS532-533.wig.gz |
