|
| Status |
Public on Nov 20, 2010 |
| Title |
S2-DRSC-CG5442-RNAi-2 |
| Sample type |
SRA |
| |
|
| Source name |
S2-DRSC-CG5442-RNAi
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cell line: S2-DRSC
|
| Treatment protocol |
Cells were treated with dsRNA to the indicated gene
|
| Growth protocol |
Schneider's medium + 10% heat-inactivated FCS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Oligo-dT selected, double stranded cDNA, Single Read Sequencing
TRIzol Dnase Rneasy
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
dsRNA treatment: CG5442
read length: 76
read length: 80
library_source: RNA
library_selection: RT and size selection
|
| Data processing |
Reads were aligned to the reference genome and a splice junction database to identify uniquely mapping reads and the data converted to SAM files and .bedgraph files which indicate the number of reads that map to each base in the genome.
|
| |
|
| Submission date |
Nov 19, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Brenton R Graveley |
| E-mail(s) |
graveley@uchc.edu
|
| Phone |
860-679-2090
|
| Organization name |
University of Connecticut Health Center
|
| Department |
Genetics and Genome Sciences
|
| Street address |
400 Farmington Avenue
|
| City |
Farmington |
| State/province |
CT |
| ZIP/Postal code |
06030-3301 |
| Country |
USA |
| |
|
| Platform ID |
GPL9061 |
| Series (1) |
| GSE18508 |
modENCODE Drosophila RNA Binding Protein RNAi RNA-Seq Studies |
|
| Relations |
| Reanalyzed by |
GSM3274714 |
| SRA |
SRX032561 |
| BioSample |
SAMN00139395 |
