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Sample GSM6311211 Query DataSets for GSM6311211
Status Public on Aug 01, 2022
Title Control_8
Sample type SRA
 
Source name Uterine Post Mortem Biopsy
Organism Bos taurus
Characteristics tissue: Uterine Post Mortem Biopsy
breed: Holstein-Freisan
Sex: Female
treatment: Control
Extracted molecule polyA RNA
Extraction protocol Uterine samples were taken, flash frozen before subsequent RNA extraction in TRIzol reagent. Total RNA concentration was quantified using the Nanodrop ND-1000 UV-Vis Spectophotometer. Quality of RNA was ascertained with the use of 2100 Agilent Bioanalyzer .
RNA libraries were prepared for sequencing using Illumina® TruSeq® Stranded mRNA Library preparation Kits
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Description s_23
Data processing Sequenced reads were trimmed for adaptor sequence and low-quality sequence, then mapped to genome using STAR (v2.6.1b)
Read counts per gene for each sample were estimated using featurCounts from the subread package (v.1.6.3).
Supplementary files format and content: Bos_taurus_UMD_3.1.1
Supplementary files format and content: Individual matrix tables with raw gene counts for every gene in a sample
 
Submission date Jul 08, 2022
Last update date Aug 01, 2022
Contact name Paul Cormican
E-mail(s) cormicp@tcd.ie, paul.cormican@teagasc.ie
Organization name Teagasc
Department Bioscience
Street address Grange
City Dunsany
ZIP/Postal code 00000000
Country Ireland
 
Platform ID GPL26012
Series (1)
GSE207735 The transcriptomic response of bovine uterine tissue is altered in response to sperm from high and low fertility bulls
Relations
BioSample SAMN29598295
SRA SRX16085571

Supplementary file Size Download File type/resource
GSM6311211_s_23_readcounts.txt.gz 119.0 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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