|
| Status |
Public on Aug 01, 2022 |
| Title |
Control_8 |
| Sample type |
SRA |
| |
|
| Source name |
Uterine Post Mortem Biopsy
|
| Organism |
Bos taurus |
| Characteristics |
tissue: Uterine Post Mortem Biopsy
breed: Holstein-Freisan
Sex: Female
treatment: Control
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Uterine samples were taken, flash frozen before subsequent RNA extraction in TRIzol reagent. Total RNA concentration was quantified using the Nanodrop ND-1000 UV-Vis Spectophotometer. Quality of RNA was ascertained with the use of 2100 Agilent Bioanalyzer .
RNA libraries were prepared for sequencing using Illumina® TruSeq® Stranded mRNA Library preparation Kits
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
s_23
|
| Data processing |
Sequenced reads were trimmed for adaptor sequence and low-quality sequence, then mapped to genome using STAR (v2.6.1b)
Read counts per gene for each sample were estimated using featurCounts from the subread package (v.1.6.3).
Supplementary files format and content: Bos_taurus_UMD_3.1.1
Supplementary files format and content: Individual matrix tables with raw gene counts for every gene in a sample
|
| |
|
| Submission date |
Jul 08, 2022 |
| Last update date |
Aug 01, 2022 |
| Contact name |
Paul Cormican |
| E-mail(s) |
cormicp@tcd.ie, paul.cormican@teagasc.ie
|
| Organization name |
Teagasc
|
| Department |
Bioscience
|
| Street address |
Grange
|
| City |
Dunsany |
| ZIP/Postal code |
00000000 |
| Country |
Ireland |
| |
|
| Platform ID |
GPL26012 |
| Series (1) |
| GSE207735 |
The transcriptomic response of bovine uterine tissue is altered in response to sperm from high and low fertility bulls |
|
| Relations |
| BioSample |
SAMN29598295 |
| SRA |
SRX16085571 |
