|
Status |
Public on Jul 12, 2012 |
Title |
tumor |
Sample type |
genomic |
|
|
Source name |
tumor
|
Organism |
Homo sapiens |
Characteristics |
cell type: chordoma tumor
|
Growth protocol |
Iscove/RPMI 1:4 containing 10% FBS (fetal bovine), 1% ITS (Insulin, Transferin, Selenium), 2 mM glutamine and 1% Pen/Strep (Penicillin/Streptomycin). Incubation was carried out at 37°C in a humidified atmosphere of 5 % CO2.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was extracted from cells or cryo tissue using the Qiagen DNA Blood Mini Kit. DNA quality and quantity was assessed using a Spectrophotometer (Microcuvette)
|
Label |
Biotin
|
Label protocol |
As per manufacturer (Affymetrix)
|
|
|
Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
|
Description |
Chordoma (TU) 14417.birdseed-v2.chp Hybridized to SNP 6.0
|
Data processing |
CEL files (generated by Affymetrix GeneChip Command Console Software version 1.1) were processed by Genotyping Console using the birdseed v2 algorithm. Genotype Call (SNP call): AA, AB, BB, NC, and NoCall; 'Signal' = Summarized signal
|
|
|
Submission date |
Nov 30, 2010 |
Last update date |
Jul 12, 2012 |
Contact name |
Carina Fischer |
E-mail(s) |
carina.fischer@medunigraz.at
|
Organization name |
ZMF
|
Street address |
Stiftingtalstr. 24
|
City |
Graz |
ZIP/Postal code |
8010 |
Country |
Austria |
|
|
Platform ID |
GPL6801 |
Series (1) |
GSE25720 |
Affymetrix SNP 6.0 array data for chordoma samples |
|