|
| Status |
Public on Jul 12, 2012 |
| Title |
chordoma cell line p5 |
| Sample type |
genomic |
| |
|
| Source name |
chordoma cell line p5
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: chordoma cell line p5
cell line: p5
|
| Growth protocol |
Iscove/RPMI 1:4 containing 10% FBS (fetal bovine), 1% ITS (Insulin, Transferin, Selenium), 2 mM glutamine and 1% Pen/Strep (Penicillin/Streptomycin). Incubation was carried out at 37°C in a humidified atmosphere of 5 % CO2.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from cells or cryo tissue using the Qiagen DNA Blood Mini Kit. DNA quality and quantity was assessed using a Spectrophotometer (Microcuvette)
|
| Label |
Biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
|
| Description |
MUG-ChorI_p5
Chrodom_III+5.birdseed-v2.chp
Hybridized to SNP 6.0
|
| Data processing |
CEL files (generated by Affymetrix GeneChip Command Console Software version 1.1) were processed by Genotyping Console using the birdseed v2 algorithm.
Genotype Call (SNP call): AA, AB, BB, NC, and NoCall; 'Signal' = Summarized signal
|
| |
|
| Submission date |
Nov 30, 2010 |
| Last update date |
Jul 12, 2012 |
| Contact name |
Carina Fischer |
| E-mail(s) |
carina.fischer@medunigraz.at
|
| Organization name |
ZMF
|
| Street address |
Stiftingtalstr. 24
|
| City |
Graz |
| ZIP/Postal code |
8010 |
| Country |
Austria |
| |
|
| Platform ID |
GPL6801 |
| Series (1) |
| GSE25720 |
Affymetrix SNP 6.0 array data for chordoma samples |
|
