|
Status |
Public on Dec 04, 2010 |
Title |
Unfetilized eggs (Cy5) vs 32-cell stage embryos (Cy3) Replicate2 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Unfetilized eggs
|
Organism |
Ciona intestinalis |
Characteristics |
Stage: Unfetilized eggs
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA from dechorionated unfertilized eggs or 32-cell stage embryos of Ciona intestinalis was extracted by acid guanidium thiocyanate-phenol-chloroform extraction method.
|
Label |
Cy5
|
Label protocol |
Total RNA was labeled with Cy3 or Cy5 using an Agilent Quick-Amp Labeling Kit (Agilent Technologies). The protocols were according to the manufacturer's instructions.
|
|
|
Channel 2 |
Source name |
32-cell stage embryos
|
Organism |
Ciona intestinalis |
Characteristics |
Stage: 32-cell stage embryos
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA from dechorionated unfertilized eggs or 32-cell stage embryos of Ciona intestinalis was extracted by acid guanidium thiocyanate-phenol-chloroform extraction method.
|
Label |
Cy3
|
Label protocol |
Total RNA was labeled with Cy3 or Cy5 using an Agilent Quick-Amp Labeling Kit (Agilent Technologies). The protocols were according to the manufacturer's instructions.
|
|
|
|
Hybridization protocol |
Hybridization and washing were performed using GE Hybridization Kit and GE Wash Pack (Agilent Technologies). The protocols were according to the manufacturer's instructions.
|
Scan protocol |
Hybridized microarrays were scanned on an Agilent Technologies G2565BA Microarray Scanner System.
|
Data processing |
The intensity of probes was extracted from scanned microarray images using Feature Extraction 10.5 software (Agilent Technologies). All algorithms and parameters used in this analysis were the default condition of the software.
|
|
|
Submission date |
Dec 02, 2010 |
Last update date |
Dec 03, 2010 |
Contact name |
Takeshi Noda |
E-mail(s) |
noda@oist.jp
|
Phone |
090-9624-2919
|
Organization name |
Okinawa Institute of Science and Technology
|
Lab |
Marine genomics unit
|
Street address |
Tancha
|
City |
Okinawa |
ZIP/Postal code |
9040412 |
Country |
Japan |
|
|
Platform ID |
GPL5576 |
Series (1) |
GSE25817 |
Comparisons of transcriptome of Ciona intestinalis between eggs and 32-cell stage embryos to identify rapidly degrading maternal mRNA |
|