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Sample GSM634306 Query DataSets for GSM634306
Status Public on Dec 04, 2010
Title Unfetilized eggs (Cy5) vs 32-cell stage embryos (Cy3) Replicate2
Sample type RNA
 
Channel 1
Source name Unfetilized eggs
Organism Ciona intestinalis
Characteristics Stage: Unfetilized eggs
Extracted molecule total RNA
Extraction protocol Total RNA from dechorionated unfertilized eggs or 32-cell stage embryos of Ciona intestinalis was extracted by acid guanidium thiocyanate-phenol-chloroform extraction method.
Label Cy5
Label protocol Total RNA was labeled with Cy3 or Cy5 using an Agilent Quick-Amp Labeling Kit (Agilent Technologies). The protocols were according to the manufacturer's instructions.
 
Channel 2
Source name 32-cell stage embryos
Organism Ciona intestinalis
Characteristics Stage: 32-cell stage embryos
Extracted molecule total RNA
Extraction protocol Total RNA from dechorionated unfertilized eggs or 32-cell stage embryos of Ciona intestinalis was extracted by acid guanidium thiocyanate-phenol-chloroform extraction method.
Label Cy3
Label protocol Total RNA was labeled with Cy3 or Cy5 using an Agilent Quick-Amp Labeling Kit (Agilent Technologies). The protocols were according to the manufacturer's instructions.
 
 
Hybridization protocol Hybridization and washing were performed using GE Hybridization Kit and GE Wash Pack (Agilent Technologies). The protocols were according to the manufacturer's instructions.
Scan protocol Hybridized microarrays were scanned on an Agilent Technologies G2565BA Microarray Scanner System.
Data processing The intensity of probes was extracted from scanned microarray images using Feature Extraction 10.5 software (Agilent Technologies). All algorithms and parameters used in this analysis were the default condition of the software.
 
Submission date Dec 02, 2010
Last update date Dec 03, 2010
Contact name Takeshi Noda
E-mail(s) noda@oist.jp
Phone 090-9624-2919
Organization name Okinawa Institute of Science and Technology
Lab Marine genomics unit
Street address Tancha
City Okinawa
ZIP/Postal code 9040412
Country Japan
 
Platform ID GPL5576
Series (1)
GSE25817 Comparisons of transcriptome of Ciona intestinalis between eggs and 32-cell stage embryos to identify rapidly degrading maternal mRNA

Data table header descriptions
ID_REF
VALUE normalized log10 ratio Cy5/Cy3

Data table
ID_REF VALUE
1 -1.23E+00
2 -2.48E-01
3 -1.22E-01
4 4.57E-02
5 -2.49E-01
6 2.12E-01
7 -1.23E+00
8 -1.47E-01
9 -5.19E-02
10 -2.62E-02
11 -1.88E-01
12 -1.18E-01
13 -1.22E-01
14 -4.21E-01
15 4.21E-01
16 -7.46E-02
17 -2.07E-01
18 -1.23E-01
19 5.08E-02
20 -4.59E-02

Total number of rows: 44290

Table truncated, full table size 657 Kbytes.




Supplementary file Size Download File type/resource
GSM634306.txt.gz 10.0 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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