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Sample GSM637871 Query DataSets for GSM637871
Status Public on Dec 10, 2010
Title SAMPLE012 null
Sample type RNA
Source name E9.5 anterior neural tube
Organism Mus musculus
Characteristics strain: LMBc
genotype/variation: Splotch null
tissue: posterior neural tube
age: embryo day 9.5
Treatment protocol The posterior part of the embryo (immediately below the forelimb pit) was cut off and transferred separately to a 600 uL plastic tube containing 15 uL of RNAlater (Ambion).
Growth protocol Embryos were removed from pregnant dams at embryonic day 9.5. At E 9.5, the average embryo was ā€œCā€ shaped and had 21.3 pairs of somites and neurul tube closure was complete with the exception of the posterior neuropore.
Extracted molecule total RNA
Extraction protocol The total RNA was extracted from collected posterior parts of E9.5 embryos using PicoPure RNA isolation Kit (Arcturus).
Label biotin-UTP
Label protocol RNA that has passed the Agilent Technologies 2100 Bioanalyzer quality control test was used to generate biotin-labeled cRNA via a modified Eberwine RNA amplification protocol
Hybridization protocol Hybridization was according Applied Microarrays Specifications. Detection of biotin label used Alexa-Fluro
Scan protocol Agilent DNA Microarray Scanner
Description 36227 oligo probes, CodeLink Mouse Whole Genome Bioarray
Data processing Initial feature extraction from the images was performed using CodeLink Expression Analysis software v4.0. Global-Median-Normalization.
Submission date Dec 09, 2010
Last update date Dec 10, 2010
Contact name Robert Cabrera
Organization name DPRI
Street address 1400 Barbara Jordan Blvd
City Austin
State/province TX
ZIP/Postal code 78723
Country USA
Platform ID GPL2897
Series (2)
GSE25975 Gene expression-based analysis of neural tube closure for the posterior neuropore of Splotch embryos at E9.5
GSE25983 Gene expression-based analysis of neural tube closure for the posterior neuropore

Data table header descriptions
VALUE Normalized Signal Intensities

Data table
301077 0.623103259
301091 74.23721193
383035 0.878307681
383049 0.85044285
383091 6.840695067
383105 16.30042389
56001 0.328626662
56015 14.77381404
56085 32.19422682
220057 13.96204025
302001 98.11164981
302029 16.53443226
302043 13.77334969
302057 30.20865108
302071 72.70731086
302085 3.970226818
302099 24.83441539
384001 19.92676258
384015 13.33995328
384029 0.119910786

Total number of rows: 36227

Table truncated, full table size 654 Kbytes.

Supplementary file Size Download File type/resource
GSM637871_T00341113_NegativeControlSummary.txt.gz 3.0 Kb (ftp)(http) TXT
GSM637871_T00341113_PositiveControlSummary.txt.gz 3.6 Kb (ftp)(http) TXT
GSM637871_T00341113_SAMPLE012.txt.gz 1.4 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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