|
| Status |
Public on Dec 10, 2010 |
| Title |
SAMPLE012 null |
| Sample type |
RNA |
| |
|
| Source name |
E9.5 anterior neural tube
|
| Organism |
Mus musculus |
| Characteristics |
strain: LMBc
genotype/variation: Splotch null
tissue: posterior neural tube
age: embryo day 9.5
|
| Treatment protocol |
The posterior part of the embryo (immediately below the forelimb pit) was cut off and transferred separately to a 600 uL plastic tube containing 15 uL of RNAlater (Ambion).
|
| Growth protocol |
Embryos were removed from pregnant dams at embryonic day 9.5. At E 9.5, the average embryo was “C” shaped and had 21.3 pairs of somites and neurul tube closure was complete with the exception of the posterior neuropore.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA was extracted from collected posterior parts of E9.5 embryos using PicoPure RNA isolation Kit (Arcturus).
|
| Label |
biotin-UTP
|
| Label protocol |
RNA that has passed the Agilent Technologies 2100 Bioanalyzer quality control test was used to generate biotin-labeled cRNA via a modified Eberwine RNA amplification protocol
|
| |
|
| Hybridization protocol |
Hybridization was according Applied Microarrays Specifications. Detection of biotin label used Alexa-Fluro
|
| Scan protocol |
Agilent DNA Microarray Scanner
|
| Description |
36227 oligo probes, CodeLink Mouse Whole Genome Bioarray
T00341113
|
| Data processing |
Initial feature extraction from the images was performed using CodeLink Expression Analysis software v4.0. Global-Median-Normalization.
|
| |
|
| Submission date |
Dec 09, 2010 |
| Last update date |
Dec 10, 2010 |
| Contact name |
Robert Cabrera |
| Organization name |
DPRI
|
| Street address |
1400 Barbara Jordan Blvd
|
| City |
Austin |
| State/province |
TX |
| ZIP/Postal code |
78723 |
| Country |
USA |
| |
|
| Platform ID |
GPL2897 |
| Series (2) |
| GSE25975 |
Gene expression-based analysis of neural tube closure for the posterior neuropore of Splotch embryos at E9.5 |
| GSE25983 |
Gene expression-based analysis of neural tube closure for the posterior neuropore |
|
