|
| Status |
Public on Dec 11, 2010 |
| Title |
Microdissected germinal center, replicate 5 |
| Sample type |
RNA |
| |
|
| Source name |
Biopsy 5
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Reactive lymph node
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Allprep extraction columns
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (2-cycle amplification)
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized O/N at 45C on Affymetrix GeneChip HG 2.0 Plus Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using Affymetrix GeneChip Scanner
|
| Description |
Gene expression data from microdissected germinal centers (reactive lymph node tissue)
|
| Data processing |
The data were analyzed using DCHIP, http://biosun1.harvard.edu/complab/dchip/
|
| |
|
| Submission date |
Dec 10, 2010 |
| Last update date |
Jul 05, 2012 |
| Contact name |
Christian Steidl |
| E-mail(s) |
csteidl@bccancer.bc.ca
|
| Phone |
1-604-675-8046
|
| Organization name |
BC Cancer Agency
|
| Department |
Pathology
|
| Lab |
Steidl lab
|
| Street address |
675 West 10th Avenue
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V5Z 1L3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE25986 |
Gene expression profiling of cell lines derived from classical Hodgkin lymphoma |
| GSE25990 |
Hodgkin lymphoma |
|
| Relations |
| Reanalyzed by |
GSM956614 |
