|
| Status |
Public on Nov 01, 2011 |
| Title |
6days_a17_3 with array type Medicago from Affymetrix |
| Sample type |
RNA |
| |
|
| Source name |
6days_a17_3
|
| Organism |
Medicago truncatula |
| Characteristics |
strain: medicago truncatula (a17)
genotype/variation: mutant (wt)
harvest date: 27-07-10
treatment name: A_euteiches_6dpi - inoculation - pathogen infection,ae 6dpi
treatment time: 6day
|
| Treatment protocol |
Name:A_euteiches_6dpi - inoculation - pathogen infection,ae 6dpi:time 6day . Factor value : WT Reference protocol or description Sample treatment protocol : Djebali et al, MPMI, 2009.
|
| Growth protocol |
roots - -In vitro condition on M medium - 16h light 8h dark 22°C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
6days_a17_3:166ng. RNeasy Qiagen kit
|
| Label |
Biotin
|
| Label protocol |
labelling Biotin direct, amplification=yes, cRNA 20 ug.
|
| |
|
| Hybridization protocol |
labelled extract quantity: 15ug
|
| Scan protocol |
GCOS, Biotin:pmt voltage 570nm,650V,laser power 1
|
| Description |
MtNF-YA1 is a transcription factor involved in the regulation of nitrogen fixing nodule organogenesis. Mtnf-ya1-1 mutant has been shown to be more resistant to Aphanomyces euteiches (Ae), a root pathogen, than the A17 wild type (WT) line. In this study we want to compare both WT and mutant plant responses, 1 and 6 days post inoculation (dpi) between WT and the Mtnf-ya1-1 mutant, in order to identify MtNF-YA1-dependent gene networks during infection. Data about non inoculated A17 control plants and infected A17 at 1 dpi have been already obtained in another experiment in which the conditions of in vitro culture of M. truncatula plants and ofA.euteiches(Ae) inoculation were exactly the same than the ones used in these experiments.
|
| Data processing |
The data were normalized with the gcrma algorithm (Irizarry et al., 2003), available in the Bioconductor package (Gentleman and Carey, 2002).
|
| |
|
| Submission date |
Dec 14, 2010 |
| Last update date |
Apr 25, 2016 |
| Contact name |
stephanie huguet |
| E-mail(s) |
huguet@evry.inra.fr
|
| Organization name |
inra
|
| Street address |
2 rue gaston cremieux
|
| City |
evry |
| ZIP/Postal code |
91000 |
| Country |
France |
| |
|
| Platform ID |
GPL4652 |
| Series (1) |
| GSE26046 |
affy_infection_medicago-Comparison of plant responses upon Aphanomyces euteiches infection in wild type (A17) line and in the Mtnf-ya1-1 mutant |
|
