|
| Status |
Public on Dec 15, 2010 |
| Title |
SUM-149shPld_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
SUM-149
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: breast cancer SUM-149
disease state: triple negative inflammatory breast carcinoma
genotype/variation: three AREG shRNA viruses (shPld)
|
| Growth protocol |
SUM-149 cells were cultured in Ham's F-12 medium supplemented with fungizone (0.5 µg/mL), gentamicin (5 µg/mL), hydrocortisone (1 µg/mL), insulin (5 µg/mL, and 5% fetal bovine serum. Sh4, shPld, and shNS were cultured in the same media, but with the addition of puromycin (1mg/ml).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with the QIAGEN Rneasy Plus kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with the Agilent Bioanylizer and the Agilent RNA 6000 Nano Kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina TotalPrep-96 RNA Amplification Kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 1
SAMPLE 3
|
| Data processing |
The data were normalized using quantile normalization with IlluminaGUI in R
|
| |
|
| Submission date |
Dec 15, 2010 |
| Last update date |
Dec 15, 2010 |
| Contact name |
Andrea Baillo |
| E-mail(s) |
andreabaillo@gmail.com
|
| Organization name |
Wayne State University
|
| Street address |
4100 John R
|
| City |
Detroit |
| ZIP/Postal code |
48224 |
| Country |
USA |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE26079 |
Genome-wide analysis gene expression in SUM-149 cells expressing AREG shRNA |
|
