|
| Status |
Public on May 08, 2013 |
| Title |
CTC_lymphocyteTCD8_HTLV_HAM |
| Sample type |
SAGE |
| Anchor |
NlaIII
|
| Tag Count |
60620 |
| Tag Length |
10 |
| |
|
| Source name |
Peripheral Blood CD8+ T Lymphocytes isolated from HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) infected individuals
|
| Organism |
Homo sapiens |
| Characteristics |
organ/tissue: peripheral blood
tissue preparation: immunomagnetic separation
cell type: CD8+ T Lymphocytes
other information: Pooled samples from HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) infected individuals
|
| Biomaterial provider |
Clinical Hospital of Faculty of Medicine of Ribeirão Preto (University of São Paulo) and Regional Blood Center of Ribeirão Preto
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol® Reagent
|
| |
|
| Description |
Peripheral blood CD8 T cells were isolated using anti-CD8 Ab-coated microbeads by passing the PBMC through a magnetic cell separation system (MACS; Miltenyi Biotec) with column type LS. CD8+ T cells were isolated to >80% purity by MACS. A pool of 4 individual samples were used.
|
| Data processing |
N/A
|
| |
|
| Submission date |
Dec 17, 2010 |
| Last update date |
May 08, 2013 |
| Contact name |
Daniel Guariz Pinheiro |
| E-mail(s) |
dgpinheiro@usp.br
|
| Phone |
+55-16-2101-9300
|
| Organization name |
Faculdade de Medicina de Ribeirão Preto (Universidade de São Paulo)
|
| Department |
Departamento de Genética
|
| Lab |
Laboratório de Genética Molecular e Bioinformática
|
| Street address |
Rua Tenente Catão Roxo, 2501
|
| City |
Ribeirão Preto |
| State/province |
São Paulo |
| ZIP/Postal code |
14051-140 |
| Country |
Brazil |
| |
|
| Platform ID |
GPL4 |
| Series (1) |
| GSE26150 |
Gene expression profiles of CD8+ T Lymphocytes isolated from HTLV-1 infected individuals |
|
