|
| Status |
Public on Feb 01, 2011 |
| Title |
wild type, adult |
| Sample type |
SRA |
| |
|
| Source name |
whole worms
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
strain: N2
developmental stage: adult
|
| Growth protocol |
C. elegans were grown at 20˚C on OP50.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from synchronized worms and small RNAs were size selected, tobacco acid pyrophosphatase (TAP) treated to remove 5’ di- and triphosphate groups, ligated to 5’ and 3’ adapters and subjected to RT-PCR.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Data processing |
Sequences were parsed and mapped to the C. elegans genome (Wormbase release WS203) using CASHX (version 2.0). R, Microsoft Excel, custom perl scripts and programs included in the CASHX software package were used for subsequent data analyses.
|
| |
|
| Submission date |
Dec 17, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Taiowa Montgomery |
| E-mail(s) |
montgomery@molbio.mgh.harvard.edu
|
| Phone |
(617) 643-3319
|
| Organization name |
Massachusetts General Hospital
|
| Department |
Molecular Biology
|
| Lab |
Ruvkun
|
| Street address |
185 Cambridge Street, CPZN-7250
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114-2790 |
| Country |
USA |
| |
|
| Platform ID |
GPL9269 |
| Series (1) |
| GSE26165 |
mut-16 and other mutator-class genes modulate 22G and 26G siRNA pathways in Caenorhabditis elegans |
|
| Relations |
| SRA |
SRX035772 |
| BioSample |
SAMN00188824 |
