|
| Status |
Public on Aug 21, 2022 |
| Title |
MKN45_vehicle_4 |
| Sample type |
SRA |
| |
|
| Source name |
MKN45
|
| Organisms |
Homo sapiens; Mus musculus |
| Characteristics |
cell line: MKN45
cell type: Gastric tumor
treatment: Vehicle
time: Day 14
|
| Treatment protocol |
When tumor was established, 359 mg/kg capecitabine orally was administered one day before the administration of 5 mg/kg ERY974 for 5 consective days in the combination group. In the monotherapy group, each agent was administered as described above. Then, tumours were collected on day 14 after ERY974 administration and used for RNA analysis.
|
| Growth protocol |
MKN45 were expanded in vitro, and inoculated into the huNOG mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested from the tumors using RNeasy mini kit (Qiagen). Total RNA was used for RNAseq.
RNA libraries for RNA-seq were prepared using TruSeq Stranded mRNA Library Prep Kit following manufacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
H7FHKDSXY_PR1750_01A0101_H1_L003
|
| Data processing |
The RNAseq reads were mapped to human (GRCh38) and mouse (GRCm38) RefSeq transcripts using bowtie 1.1.2
Reads mapped to the transcripts of both organisms were excluded
The calculation of fragments per kilobase of transcript per million reads mapped (FPKM) using RSEM v1.2.31
Assembly: GRCh38 + CRCm38 with RefSeq annotations
Supplementary files format and content: tab-delimited text files include FPKM values for each Sample
|
| |
|
| Submission date |
Aug 16, 2022 |
| Last update date |
Aug 21, 2022 |
| Contact name |
YUJI SANO |
| E-mail(s) |
sanoyuj@chugai-pharm.co.jp
|
| Phone |
+810467476241
|
| Organization name |
Chugai Pharmaceutical Co., Ltd
|
| Street address |
200
|
| City |
Kajiwara |
| State/province |
Kamakura |
| ZIP/Postal code |
247-8530 |
| Country |
Japan |
| |
|
| Platform ID |
GPL25526 |
| Series (1) |
| GSE211373 |
RNA data of MKN45 tumours treated with ERY974 and/or capecitabine in huNOG mice |
|
| Relations |
| BioSample |
SAMN30348333 |
| SRA |
SRX17102624 |
