|
| Status |
Public on Dec 30, 2010 |
| Title |
Chip03_UPO_070637 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Eutopic endometrium tissue RNA
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
tissue: eutopic endometrium
disease state: ovarian endometriosis
disease stage: III
patient: 2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
MicroRNAs were extracted from tissues with the miRNeasy kit (Qiagen, Valencia, CA, USA) according to the manufacturer's protocol and quantified by the QuantiT RNA Kit with Qubit Fluorometer (Invitrogen, Carlsbad, CA, USA).
|
| Label |
Cy3
|
| Label protocol |
Performed by LC Sciences (http://www.lcsciences.com/).
|
| |
|
| Channel 2 |
| Source name |
Ectopic endometrium tissue RNA
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
tissue: ectopic endometrium
disease state: ovarian endometriosis
disease stage: III
patient: 2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
MicroRNAs were extracted from tissues with the miRNeasy kit (Qiagen, Valencia, CA, USA) according to the manufacturer's protocol and quantified by the QuantiT RNA Kit with Qubit Fluorometer (Invitrogen, Carlsbad, CA, USA).
|
| Label |
Cy5
|
| Label protocol |
Performed by LC Sciences (http://www.lcsciences.com/).
|
| |
|
| |
| Hybridization protocol |
Hybridized to the LC Sciences miRNA microarray (miRBase v9.1) overnight at 34°C. Performed by LC Sciences (http://www.lcsciences.com/).
|
| Scan protocol |
Scanned using the GenePix 4000B scanner. Fluorescent images were digitized using Array-Pro image analysis. Performed by LC Sciences (http://www.lcsciences.com/).
|
| Data processing |
All of the statistical analyses were performed by LC Sciences (http://www.lcsciences.com/) using MeV software (http://www.tm4.org/mev.html). Normalization of the samples was performed using a global normalization approach (LOWESS).
|
| |
|
| Submission date |
Dec 29, 2010 |
| Last update date |
Dec 30, 2010 |
| Contact name |
Ilaria Gregnanin |
| E-mail(s) |
ilaria.gregnanin@med.unipmn.it
|
| Organization name |
Università del Piemonte Orientale
|
| Street address |
V. Solaroli 17
|
| City |
Novara |
| ZIP/Postal code |
28100 |
| Country |
Italy |
| |
|
| Platform ID |
GPL7372 |
| Series (1) |
| GSE26346 |
Differential expression of microRNAs between eutopic and ectopic endometrium in ovarian endometriosis |
|
