|
Status |
Public on Feb 02, 2012 |
Title |
U937_TPA_48h_rep1 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
U937 cells treated with TPA
|
Organism |
Homo sapiens |
Characteristics |
tissue: acute myeloid leukemia cell line: U937
|
Treatment protocol |
Before microarray analysis,differentiation of U937 cells was induced by adding TPA (Sigma-Aldrich, St. Louis, USA) to a final concentration of 30 nM to the culture media and incubating the cells for 48 h.
|
Growth protocol |
All cells were cultured in a sterile incubator at 37˚C
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA samples were extracted from normal U937 cells and differentiated U937 cells using Trizol reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions.
|
Label |
cy5
|
Label protocol |
Fluorescent dye (Cy5 and Cy3-dCTP,ambion) labeled DNA was produced through Eberwine's linear RNA amplification method and subsequent enzymatic reaction. We took a cDNA labeling approach with Klenow enzyme after reverse transcription.
|
|
|
Channel 2 |
Source name |
U937 cells
|
Organism |
Homo sapiens |
Characteristics |
tissue: acute myeloid leukemia cell line: U937
|
Treatment protocol |
Before microarray analysis,differentiation of U937 cells was induced by adding TPA (Sigma-Aldrich, St. Louis, USA) to a final concentration of 30 nM to the culture media and incubating the cells for 48 h.
|
Growth protocol |
All cells were cultured in a sterile incubator at 37˚C
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA samples were extracted from normal U937 cells and differentiated U937 cells using Trizol reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions.
|
Label |
cy3
|
Label protocol |
Fluorescent dye (Cy5 and Cy3-dCTP,ambion) labeled DNA was produced through Eberwine's linear RNA amplification method and subsequent enzymatic reaction. We took a cDNA labeling approach with Klenow enzyme after reverse transcription.
|
|
|
|
Hybridization protocol |
standard CapitalBio Methods
|
Scan protocol |
Luxscan 10K (CapitalBio)
|
Description |
differentiation (treated ) vs normal(control)
|
Data processing |
After background correction and removal of bad spots, a space and intensity-dependent normalization based on a LOWESS program was employed.
|
|
|
Submission date |
Dec 30, 2010 |
Last update date |
Feb 02, 2012 |
Contact name |
jing wang |
E-mail(s) |
jwang.tsinghua@gmail.com
|
Organization name |
Medical Systems Biology Research Center School of Medicine Tsinghua University
|
Street address |
Tsinghu University
|
City |
Beijing |
ZIP/Postal code |
100084 |
Country |
China |
|
|
Platform ID |
GPL8380 |
Series (2) |
GSE26377 |
Microarray profiling of monocytic differentiaion reveals miRNA-mRNA intrinsic correlation (gene expression) |
GSE26379 |
Microarray profiling of monocytic differentiaion reveals miRNA-mRNA intrinsic correlation |
|