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Sample GSM647525 Query DataSets for GSM647525
Status Public on Feb 02, 2012
Title U937_TPA_48h_rep1
Sample type RNA
 
Channel 1
Source name U937 cells treated with TPA
Organism Homo sapiens
Characteristics tissue: acute myeloid leukemia
cell line: U937
Treatment protocol Before microarray analysis,differentiation of U937 cells was induced by adding TPA (Sigma-Aldrich, St. Louis, USA) to a final concentration of 30 nM to the culture media and incubating the cells for 48 h.
Growth protocol All cells were cultured in a sterile incubator at 37˚C
Extracted molecule total RNA
Extraction protocol Total RNA samples were extracted from normal U937 cells and differentiated U937 cells using Trizol reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions.
Label cy5
Label protocol Fluorescent dye (Cy5 and Cy3-dCTP,ambion) labeled DNA was produced through Eberwine's linear RNA amplification method and subsequent enzymatic reaction. We took a cDNA labeling approach with Klenow enzyme after reverse transcription.
 
Channel 2
Source name U937 cells
Organism Homo sapiens
Characteristics tissue: acute myeloid leukemia
cell line: U937
Treatment protocol Before microarray analysis,differentiation of U937 cells was induced by adding TPA (Sigma-Aldrich, St. Louis, USA) to a final concentration of 30 nM to the culture media and incubating the cells for 48 h.
Growth protocol All cells were cultured in a sterile incubator at 37˚C
Extracted molecule total RNA
Extraction protocol Total RNA samples were extracted from normal U937 cells and differentiated U937 cells using Trizol reagent (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions.
Label cy3
Label protocol Fluorescent dye (Cy5 and Cy3-dCTP,ambion) labeled DNA was produced through Eberwine's linear RNA amplification method and subsequent enzymatic reaction. We took a cDNA labeling approach with Klenow enzyme after reverse transcription.
 
 
Hybridization protocol standard CapitalBio Methods
Scan protocol Luxscan 10K (CapitalBio)
Description differentiation (treated ) vs normal(control)
Data processing After background correction and removal of bad spots, a space and intensity-dependent normalization based on a LOWESS program was employed.
 
Submission date Dec 30, 2010
Last update date Feb 02, 2012
Contact name jing wang
E-mail(s) jwang.tsinghua@gmail.com
Organization name Medical Systems Biology Research Center School of Medicine Tsinghua University
Street address Tsinghu University
City Beijing
ZIP/Postal code 100084
Country China
 
Platform ID GPL8380
Series (2)
GSE26377 Microarray profiling of monocytic differentiaion reveals miRNA-mRNA intrinsic correlation (gene expression)
GSE26379 Microarray profiling of monocytic differentiaion reveals miRNA-mRNA intrinsic correlation

Data table header descriptions
ID_REF
VALUE lowess normalized log2 ratio(differentiation/normal)

Data table
ID_REF VALUE
1 -0.2007
2 -0.1579
3 -0.7755
4 0.4213
5 -9.8283
6 0.7128
7 -1.3880
8 -1.4259
9 -1.5199
10 -1.0770
11 -1.0321
12 1.4550
13 -0.9768
14 -1.9105
15 0.4333
16 1.4990
17 1.8588
18 -0.2149
19 -0.0544
20 1.8095

Total number of rows: 23232

Table truncated, full table size 294 Kbytes.




Supplementary file Size Download File type/resource
GSM647525.LSR.gz 1.5 Mb (ftp)(http) LSR
Processed data included within Sample table

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