|
| Status |
Public on Jul 19, 2023 |
| Title |
BJAB_IRF4C99R_48hrs_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
48-hrs Dox Tet-inducible IRF4C99R transfected BJAB cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: BJAB
group: IRF4C99R 48hrs
|
| Treatment protocol |
Cells were transfected with Tet-inducible control, WT or C99R IRF4 and treated with doxycycline for 0, 6, 24 or 48 hrs
|
| Growth protocol |
Cells were grown at 37°C with 5% CO2 in RPMI-1640 medium with 10% FCS, 100 units/ml penicillin, 100 ?g/ml streptomycin and 2 mM glutamine at a density of 1-5 105 cells/ml
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cells using the Qiagen RNeasy kit according to manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotynilation of cRNA was carried out using the Ambion Prep kit for Illumina BeadChip arrays
|
| |
|
| Hybridization protocol |
Hybridization was performed for 16 hours at 58°C per Illumina instructions
|
| Scan protocol |
BeadChip scanning was performed using SentrixScan
|
| Description |
BJ1x48hD2
|
| Data processing |
Intensity normalisation was carried out in Illumina GenomeStudio and differential expression analysis using limma
|
| |
|
| Submission date |
Aug 23, 2022 |
| Last update date |
Jul 19, 2023 |
| Contact name |
Pierre Daniel Cauchy |
| E-mail(s) |
cauchy@ie-freiburg.mpg.de
|
| Phone |
+49 (0)761 270-77576
|
| Organization name |
University Medical Center Freiburg
|
| Department |
Zentrum für Translationale Zellforschung
|
| Lab |
AG Onco-Immunology
|
| Street address |
Breisacher Str. 115
|
| City |
Freiburg |
| State/province |
Baden-Württemberg |
| ZIP/Postal code |
79106 |
| Country |
Germany |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE211445 |
C99R mutation in IRF4 drives a novel gain of function binding and gene upregulation in classical Hodgkin lymphoma |
| GSE211913 |
C99R mutation in IRF4 drives a novel gain of function binding and gene upregulation in classical Hodgkin lymphoma [Illumina BeadChip] |
|
