cell type: lymphocytes sex: male disease status: unaffected inheritance: recessive
Extracted molecule
genomic DNA
Extraction protocol
Standard salting out dna extraction protocol using PureGene reagents
Label
biotin
Label protocol
Standard Affymetrix 6.0 labeling protocol
Hybridization protocol
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
Scan protocol
Array was washed using the Affymetrix fluidics stations and scanned on Affymetrix 3000-7G scanner.
Data processing
Data was collected and processed using the Genotyping console software. Genotypes were called with Birdseed v2.
primary data description (complete table provided as a supplementary file on the Series record): Chromosome 6 (126000054-140001863) copy number analysis results for 86828. Sample called with a European reference file. ENPP1 position is 132,170,849-132,257,988 (hg18). Datafields for the table are ProbeSet, Chromosome, Chromosomal Position, CNState, Log2Ratio, SmoothSignal, LOH, Allele Difference, dbSNP RSID
