|
| Status |
Public on Oct 06, 2022 |
| Title |
Hv_nsp2_+P+N_rep3 |
| Sample type |
SRA |
| |
|
| Source name |
whole root
|
| Organism |
Hordeum vulgare |
| Characteristics |
tissue: whole root
genotype: nsp2-1 mutant in Golden Promise background
treatment: +P+N
time: 21 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
H. vulgare total RNA was extracted using the Spectrum™ Plant Total RNA kit (SigmaAldrich) and M. truncatula total RNA was isolated with the RNeasy PlantMini Kit (Qiagen), following the manufacturer’s instructions.
Poly A enrichment
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
rawcounts_Hv.tsv
|
| Data processing |
Quality control was conducted with FastQC software
Resultant raw fastq data were mapped to the M. truncatula reference genome version 4.0 or the H. vulgare cv. Golden Promise reference genome with STAR software
The raw count values were calculated with featureCounts in R package Rsubread
Assembly: Mt4.0v1, Hordeum vulgare cv. Golden Promise
Supplementary files format and content: tab separated files with raw count values
|
| |
|
| Submission date |
Oct 03, 2022 |
| Last update date |
Oct 06, 2022 |
| Contact name |
Darius Zarrabian |
| E-mail(s) |
dtz21@cam.ac.uk
|
| Organization name |
Crop Science Centre, University of Cambridge
|
| Department |
Plant Sciences
|
| Street address |
93 Lawrence Weaver Rd
|
| City |
Cambridge |
| ZIP/Postal code |
CB3 0LE |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL29652 |
| Series (1) |
| GSE214698 |
Nutrient regulation of lipochitooligosaccharide recognition in plants via NSP1 and NSP2 |
|
| Relations |
| BioSample |
SAMN31139020 |
| SRA |
SRX17781908 |
