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Status |
Public on Jul 04, 2011 |
Title |
OPM2 DMSO Control |
Sample type |
RNA |
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Source name |
B lymphocyte
|
Organism |
Homo sapiens |
Characteristics |
cell type: Multiple Myeloma Cell Line cell line: OPM-2 treatment group: DMSO Control
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Treatment protocol |
Cells were treated with 0.5 μmol/L DZNep for 48 h.
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Growth protocol |
Human MM cell lines KMS18, MM.1S and OPM-2 were maintained in RPMI 1640, supplemented with 10% fetal calf serum (FCS), 100 U/mL penicillin and 100 ug/mL streptomycin. MM cell line NCI-H929 was cultured in RPMI 1640 with 15% FCS and 0.00036% 2-mercaptoethanol. All cells were grown at 37°C in a humidified atmosphere with 5% CO2.
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen RNeasy Mini kit (Germany) extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated aRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (GeneChip 3' IVT Express Kit Manual, 2009, Affymetrix).
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|
|
Hybridization protocol |
Following fragmentation, 12.5 ug of aRNA were hybridized for 16 hr at 45C on GeneChip U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
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Scan protocol |
GeneChips were scanned using the Affymetrix scanner 3000 7G
|
Description |
7.OPM-2 DMSO OPM2-DMSO
|
Data processing |
The data were analyzed with Genespring GX (Agilent Technologies). The data normalization was done using MAS5 algorithm in GeneSpring GX. For each control-treatment comparison, entities that have flagged present or marginal in at least 1 out of 2 samples were retained, and the differentially expressed genes were identified using the fold change function.
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|
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Submission date |
Jan 27, 2011 |
Last update date |
Jul 04, 2011 |
Contact name |
Zhigang Xie |
E-mail(s) |
xiezhigang@nus.edu.sg
|
Organization name |
National University of Singapore
|
Department |
CSI Singapore
|
Street address |
14 Medical Drive, CeLS Building #02-07
|
City |
Singapore |
ZIP/Postal code |
117599 |
Country |
Singapore |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE26921 |
Determinants of sensitivity to DZNep induced apoptosis in multiple myeloma cells |
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