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| Status |
Public on Jun 01, 2011 |
| Title |
JMM-cel-020_ego-1_fem-1_L4_mRNA-2 |
| Sample type |
SRA |
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| Source name |
L4 hermaphrodite
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| Organism |
Caenorhabditis elegans |
| Characteristics |
genotype: ego-1(om84) unc-29(e193) fem-1(hc17)
strain: PD8826
rna-seq method: mRNAseq
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| Extracted molecule |
polyA RNA |
| Extraction protocol |
High molecular weight RNA (Ambion - miRvana) was polyA+ selected (Ambion - PolyApurist). We then fragmented the polyA with RNA fragmentation reagents (Ambion) and treated the RNA with T4 polynucleotide kinase. These treated RNAs were then ligated to a 3' adapter (IDT-Linker-1 or IDT-Linker-2). These 3'-captured fragmented RNAs were size selected and ligated to a barcoded 5' adapter, reverse transcribed, amplified and sequenced.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina Genome Analyzer II |
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| Description |
barcode: TGAA
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| Data processing |
Raw data only (no processing performed).
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| Submission date |
Feb 02, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Jay Maniar |
| E-mail(s) |
jmmaniar@stanford.edu
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| Phone |
6509062841
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| Fax |
6507256902
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| Organization name |
Stanford University
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| Department |
Genetics
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| Lab |
Fire
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| Street address |
300 Pasteur Dr L302
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platform ID |
GPL9269 |
| Series (2) |
| GSE26579 |
mRNA sequencing from ego-1(om84) and control L3, L4, and adult C. elegans hermaphrodites |
| GSE26582 |
EGO-1, an essential C. elegans RdRP, modulates gene expression through the mRNA-templated production of short antisense effector RNAs |
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| Relations |
| SRA |
SRX040319 |
| BioSample |
SAMN00203955 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data not provided for this record |
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