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| Status |
Public on Jun 01, 2011 |
| Title |
JMM-cel-001_ego-1_fem-1_L3_5'INDEP-1 |
| Sample type |
SRA |
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| Source name |
L3 hermaphrodite
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| Organism |
Caenorhabditis elegans |
| Characteristics |
genotype: ego-1(om84) unc-29(e193) fem-1(hc17)
strain: PD8826
rna-seq method: 5'AP-PNK-sRNAseq
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| Extracted molecule |
total RNA |
| Extraction protocol |
Small RNAs were size-selected (Ambion - miRvana). We then captured the 3' end (IDT-Linker-1 or IDT-Linker-2). These 3'-captured small RNAs were then further size selected and treated first with antarctic phosphatase and T4 polynucleotide kinase. Subsequently, these treated RNAs were ligated to a barcoded 5' adapter, reverse transcribed, amplified and sequenced.
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| Library strategy |
ncRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina Genome Analyzer II |
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| Description |
barcode: TTCT
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| Data processing |
Raw data only (no processing performed).
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| Submission date |
Feb 02, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Jay Maniar |
| E-mail(s) |
jmmaniar@stanford.edu
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| Phone |
6509062841
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| Fax |
6507256902
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| Organization name |
Stanford University
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| Department |
Genetics
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| Lab |
Fire
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| Street address |
300 Pasteur Dr L302
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platform ID |
GPL9269 |
| Series (2) |
| GSE26581 |
5'-monophosphate-independent small RNA sequencing from ego-1(om84) and control L3, L4, and adult C. elegans hermaphrodites |
| GSE26582 |
EGO-1, an essential C. elegans RdRP, modulates gene expression through the mRNA-templated production of short antisense effector RNAs |
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| Relations |
| SRA |
SRX040339 |
| BioSample |
SAMN00203975 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data not provided for this record |
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