|
| Status |
Public on Jan 01, 2013 |
| Title |
Normal_rat12454 |
| Sample type |
RNA |
| |
|
| Source name |
Normal
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Fischer 344
age: 5 months
gender: male
tissue: bone - right ulna (central 5 mm)
microarray chip: 2
sentrix position: E
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
RNA transcripts were amplified by T7 linear amplification (MessageAmp TotalPrep amplification kit; ABI-Ambion
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 1
|
| Data processing |
The data were normalized using quantile normalization with Illumina BeadStudio software (Illumina Beadscan, v3)
|
| |
|
| Submission date |
Feb 04, 2011 |
| Last update date |
Jan 01, 2013 |
| Contact name |
Jennifer McKenzie |
| E-mail(s) |
mckenziej@wudosis.wustl.edu
|
| Phone |
3144548198
|
| Fax |
3143620334
|
| Organization name |
Washington University in St. Louis
|
| Department |
Orthopedic Surgery
|
| Lab |
Silva Lab
|
| Street address |
660 S. Euclid, Box 8233
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL6101 |
| Series (1) |
| GSE27065 |
Differential gene expression from microarray analysis distinguishes woven and lamellar bone formation in the rat ulna following mechanical loading |
|
