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Sample GSM6689691 Query DataSets for GSM6689691
Status Public on Jan 01, 2024
Title Non-L-pipecolic acid producing LPA-0 C.
Sample type RNA
 
Source name modified Corynebacterium glutamicum ATCC13032 (LPA-0)
Organism Corynebacterium glutamicum
Characteristics phenotype: OD4 non-L-pipecolic acid producing
Treatment protocol cells (500 µL) were harvested via centrifugation (30 sec, 4°C, 13.000 x g) and snap-frozen in liquid nitrogen
Growth protocol baffeled 500 mL shake flask, 50 mL potassium based minimal medium (10 g L-1 glucose), 30°C, 230 rpm, initial OD660 = 0.2
Extracted molecule total RNA
Extraction protocol mRNA extracted from harvested cell pellets (6 mg DCW) using Qiagen RNeasy kit and Turbo DNAfree kit
Label Cy3
Label protocol Agilent labeling kit (one color)
 
Hybridization protocol Agilent hybridisation kit
Scan protocol Agilent SureScan S2600D Microarray Scanner
Description cell biomass
exponential growth
LPA-0 C
Data processing GeneSpring 14.9 GC, Feature extraction, Statistical analysis (moderated t-test, p_corr < 0.05 (Benjamini-Hochberg), 2fold change), export
 
Submission date Oct 27, 2022
Last update date Jan 01, 2024
Contact name Michael Kohlstedt
E-mail(s) michael.kohlstedt@uni-saarland.de
Organization name Saarland University
Street address Campus A1.5
City Saarbruecken
ZIP/Postal code 66123
Country Germany
 
Platform ID GPL32785
Series (2)
GSE216735 Gene expression in glucose-grown L-pipecolic acid production strains C.glutamicum LPA-1A and LPA-5 compared to the non-L-pipecolic acid-producing strain C. glutamicum LPA-0 [2]
GSE216736 Gene expression in glucose-grown L-pipecolic acid production strains C.glutamicum LPA-1A and LPA-5 compared to the non-L-pipecolic acid-producing strain C. glutamicum LPA-0

Supplementary file Size Download File type/resource
GSM6689691_LPA-0_C.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data are available on Series record

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