|
| Status |
Public on Jan 01, 2024 |
| Title |
L-pipecolic acid producing LPA-5 B. |
| Sample type |
RNA |
| |
|
| Source name |
modified Corynebacterium glutamicum ATCC13032 (LPA-5)
|
| Organism |
Corynebacterium glutamicum |
| Characteristics |
phenotype: OD4 higher L-pipecolic acid producing
|
| Treatment protocol |
cells (500 µL) were harvested via centrifugation (30 sec, 4°C, 13.000 x g) and snap-frozen in liquid nitrogen
|
| Growth protocol |
baffeled 500 mL shake flask, 50 mL potassium based minimal medium (10 g L-1 glucose), 30°C, 230 rpm, initial OD660 = 0.2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mRNA extracted from harvested cell pellets (6 mg DCW) using Qiagen RNeasy kit and Turbo DNAfree kit
|
| Label |
Cy3
|
| Label protocol |
Agilent labeling kit (one color)
|
| |
|
| Hybridization protocol |
Agilent hybridisation kit
|
| Scan protocol |
Agilent SureScan S2600D Microarray Scanner
|
| Description |
cell biomass
exponential growth
LPA-5 B
|
| Data processing |
GeneSpring 14.9 GC, Feature extraction, Statistical analysis (moderated t-test, p_corr < 0.05 (Benjamini-Hochberg), 2fold change), export
|
| |
|
| Submission date |
Oct 27, 2022 |
| Last update date |
Jan 01, 2024 |
| Contact name |
Michael Kohlstedt |
| E-mail(s) |
michael.kohlstedt@uni-saarland.de
|
| Organization name |
Saarland University
|
| Street address |
Campus A1.5
|
| City |
Saarbruecken |
| ZIP/Postal code |
66123 |
| Country |
Germany |
| |
|
| Platform ID |
GPL32785 |
| Series (2) |
| GSE216735 |
Gene expression in glucose-grown L-pipecolic acid production strains C.glutamicum LPA-1A and LPA-5 compared to the non-L-pipecolic acid-producing strain C. glutamicum LPA-0 [2] |
| GSE216736 |
Gene expression in glucose-grown L-pipecolic acid production strains C.glutamicum LPA-1A and LPA-5 compared to the non-L-pipecolic acid-producing strain C. glutamicum LPA-0 |
|
